Author(s):
1. NASREEN IJAZ:
Fisheries Research and Training Institute, Manawan, Lahore (NI), Pakistan
2. ZAFAR IQBAL:
Department of Zoology University of the Punjab, Lahore (ZI), Pakistan
3. JAMIL ANWAR CHAUDHRY:
Institute of Chemistry, University of the Punjab, Lahore (JAC), Pakistan
Abstract:
Experiments were conducted to establish the lethal dose concentration of un-ionized ammonia at which 50% and 100% mortality of fingerlings of Ctenopharyngodon idella (Valenciennes) occurred. Fingerlings of C. idella were obtained from Central Fish Seed Hatchery, Lahore and were stocked in 300 liters cemented indoor rectangular tanks. The tanks were supplied tube-well water (temperature 13-19 0C, pH 7.98.8; hardness 276-344 mg/l as CaCO3, dissolved oxygen 5.0-8.1ppm with zero total ammonia nitrogen. Fingerlings were fed daily with standard fish feed. Median lethal concentrations (LC50) were determined in glass aquaria each containing 9 fish (mean weight 12.7±1.4 to 7.2±1.31g) and 40 liters of test water. Total ammonia-N concentration was determined by direct Nesslerization. The 24 hour LC50 of un-ionized ammonia for C. idella fingerlings (mean body weight 2.50 ± 1.54g, at 15±0.10C and pH 8.0 ± 0.01) was 0.05mg/l. Lethal dose of un-ionized ammonia at which 100% C. idella fingerlings (mean body weight 2.96 ±0.8g, at 15±0.10C and pH 8.12 ± 0.02) showed mortality, was 0.075mg/l. Fish under experiment remained unharmed when ammonia concentration was 0.025 mg/l.
Page(s):
9-20
DOI:
DOI not available
Published:
Journal: Punjab University Journal of Zoology, Volume: 26, Issue: 1, Year: 2011
Keywords:
Toxicity
,
LC50
,
Ammonia
,
LC100
,
fish survival
References:
References are not available for this document.
Citations
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