A Rapid Microtiter Assay to Evaluate Fungicide Sensitivity to Colletotrichum falcatum Isolates | [International Journal of Agriculture and Biology • 2022]

Author(s):

1. Renato de Carvalho Menezes: Universidade Federal de Goiás, Goias, Brazil

2. Mariana Guimarães Silva: Universidade Federal de Goiás, Goias, Brazil

3. Thayssa Monize Rosa Oliveira,: Universidade Federal de Goiás, Goias, Brazil

4. Geisiane Alves Rocha: Universidade Federal de Goiás, Goias, Brazil

5. Vanessa Duarte Dias: Universidade Federal de Goiás, Goias, Brazil

6. Renato Carrer Filho: Universidade Federal de Goiás, Goias, Brazil

7. Marcos Gomes da Cunha: Universidade Federal de Goiás, Goias, Brazil

Abstract:

Chemical control of sugarcane red rot, caused by Colletotrichum falcatum, forms part of integrated management of the disease. A rapid microtiter bioassay based on the colorimetric changes of resazurin dye was developed to evaluate the sensitivity of C. falcatum to the main chemical fungicide groups, including strobilurin, triazole, benzimidazole, isophthalonitrile and dithiocarbamate. There was no significant difference among the isolates in terms of growth inhibition for any of the active ingredients tested (a = 0.01). The C. falcatum isolates showed almost similar sensitivity to various fungicides. The active ingredients varied in relation to fungitoxicity. Doses that inhibited 50% of C. falcatum growth were calculated as a percentage of resazurin reduction due to various fungicides. The colorimetric method used to assess the fungitoxicity of active ingredients to C. falcatum, combined with resazurin, proved a fast practical and efficient method.

Page(s): 83-88

DOI: 10.17957/IJAB/15.1902

Published: Journal: International Journal of Agriculture and Biology, Volume: 27, Issue: 1, Year: 2022

Keywords:

Sugarcane , red rot , fungicide , chemical control , Resazurin , Fungitoxicity

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