Antimicrobial Effects of Silver-phyconanoparticles from Sargassum Vulgare against Spoilage of Fresh Vegetables Caused by Bacillus Cereus, Fusarium solani and Alternaria alternata. | International Journal of Agriculture and Biology 2018

Fresh vegetables production in Mexico is adversely affected by fungal and bacterial diseases associated with Fusarium solani, Alternaria alternata and Bacillus cereus that are associated with postharvest decay and diarrheal syndrome. Hence, this study was conducted to investigate the synthesis and characterization of AgNPs using the aqueous extract of Sargasssum vulgare. Antimicrobial activities of biosynthesized AgNPs were evaluated against B. cereus, F. solani and A. alternata to demonstrate the bionanotechnological potentialities of these brown seaweeds. The UV-visible spectroscopic analysis showed the absorbance peaked at 460 nm, which indicated the synthesis of silver nanoparticles. The zeta potential of the biosynthesized AgNPs was found as a sharp peak at 27.5 mV with polydispersity index of 2.17 and polarity negative, while the mean particle size was 21.18 nm. The results of dual plate assays revealed that AgNPs showed broad spectrum antagonism (p = 0.05) against F. solani (70.9%) and A. alternata (55.05%) after nine days of incubation. On the other hand, different concentrations of AgNPs (25, 50, 75 and 100 mg/mL) showed antibacterial activity against B. cereus in comparison with the control after 24 h of incubation. Finally, further studies are required to confirm their potential of AgNPs from S. vulgare in the control of the F. solani, A. alternata and B. cereus under field conditions.

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