Utilization of biological control agents for the management of postharvest pathogens of tomato. | [Pakistan Journal of Botany • 2016]

Author(s):

1. Muhammad Usman Ghazanfar: Department of Plant Pathology, University College of Agriculture, University of Sargodha, Sargodha, Pakistan; Biosafety Laboratory, Reckenholzstrasse 191, CH-8046 Zürich, Switzerland

2. Muzammil Hussain: Department of Plant Pathology, University College of Agriculture, University of Sargodha, Sargodha, Pakistan; Biosafety Laboratory, Reckenholzstrasse 191, CH-8046 Zürich, Switzerland

3. Muhammad Imran Hamid: Department of Plant Pathology, University College of Agriculture, University of Sargodha, Sargodha, Pakistan; Biosafety Laboratory, Reckenholzstrasse 191, CH-8046 Zürich, Switzerland

4. Sami Ullah Ansari: Department of Applied Statistics, University College of Agriculture, University of Sargodha, Sargodha, Pakistan

Abstract:

Twenty five isolates of Trichoderma, Bacillus and Pseudomonas spp. were obtained from rhizosphere of tomato growing fields using soil dilution technique on potato dextrose agar (PDA) and nutrient agar (NA) medium. Screening of these isolates were done against Geotrichum candidum, Trichothecium roseum and Rhizopus oryzae, causal agents of sour rot, pink mold rot and Rhizopus soft rot of tomato under the laboratory conditions. One promising isolate of each Trichoderma harzianum, Bacillus spp. and Pseudomonas fluorescens from the twenty five isolates were chosen and further evaluated as potential biological control agents (BCAs) against three important postharvest pathogens of tomato. Dual culture and spore concentration assay revealed that all three isolates inhibited radial growth of G. candidum, T. roseum and R. oryzae. Tomato fruits were inoculated with 25μl suspension of l08 cfu mL-1 for T. harzianum and l08cfu mL-1for each Bacillus sp. and P.fluorescens. Twenty four hours later the treated fruits were inoculated with 25μl of 105 conidia/mL of each of three postharvest pathogens. The results showed that P. fluorescens provided good control (78.1%) of G. candidum and (82.2%) R. oryzae, while, T. harzianum proved less effective to control all three pathogens. Bacillus spp. was only effective (88.4%) against T. roseum. Hence, our results depicted that Bacillus spp. and P. fluorescens proved to be a potential antagonist of T. roseum and R. oryzae however, all the tested BCAs were not consistent in their action against three postharvest pathogens of tomato.

Page(s): 2093-2100

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 48, Issue: 5, Year: 2016

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