The antiviral mechanism of the crude extract from the flowers of Trollius chinensis based on TLR 3 signaling pathway | [Pakistan Journal of Pharmaceutical Sciences • 2021]

Author(s):

1. Li-jia Liu: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

2. Del-i Li: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

3. Ming-yue Fang: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

4. Shuang-yue Liu: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

5. Qing-qing Wang: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

6. Yu-xi Liang: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

7. Xiu-hua Hu: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

8. Ru-feng Wang: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

Abstract:

The effects of crude extract from the flowers of Trollius chinensis on expressions of mRNA and proteins related to vital genes (TLR 3, TBK 1, IRF 3 and IFN ß) in TLR 3 signaling pathway were investigated in the presence/absence of Polyinosinic acid-polycytidylic acid (PolyI: C) to ascertain the antiviral mechanism of these flowers. Real-time PCR and western blot were applied to determine the expressions of mRNA and proteins, respectively, and immunofluorescence assay was employed to study the effect on IRF 3 distribution between nuclei and cytoplasma. In the absence of PolyI:C, the crude extract reduced the mRNA expression of TLR 3, IRF 3 and IFN ß and the protein expression of TLR 3, and increased the protein expression of IRF 3 and the distribution of IRF 3 in nuclei. In the presence of PolyI:C, the extract reduced the mRNA and protein expressions of TLR 3 and the mRNA expression of IFN ß, meanwhile inhibited the translocation of IRF 3 into nuclei. The antiviral mechanism of the crude extract from the flowers of T. chinensis is to protect the host from inflammatory damage through intervening the TLR 3 signaling pathway and reducing the secretion of inflammatory factors.

Page(s): 1743-1748

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 34, Issue: 5, Year: 2021

Keywords:

mRNA , antiviral mechanism , TLR 3 signaling pathway , Trollius chinensis

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