Application of Natural Media for Isolation and Culturing of Oligodendrocyte Precursor Cells from Neonatal Quail Brain | First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book) 2022

Author(s)

Mouhamed, Z. K. Department of Biosciences, COMSATS University Islamabad,Pakistan

Adissa Raimi Department of Biosciences, COMSATS University Islamabad,Pakistan

Rida Azmat Department of Biosciences, COMSATS University Islamabad, Pakistan

HiraAkmal Department of Biosciences, COMSATS University Islamabad, Pakistan

Asghar Shabbir Department of Biosciences, COMSATS University Islamabad, Pakistan

Abstract

Oligodendrocytes are myelin forming cells in the central nervous system (CNS). They also provide metabolic support to neurons which they cover. Oligodendrocyte death leads to demyelination and hence myelin associated disorders like multiple sclerosis. In vitro studies, involving isolation and growth of oligodendrocyte precursor cells (OPCs) provide new insight related to neuron-oligodendrocyte interaction, demyelination, remyelination, proliferation and differentiation etc. In the present study we have used a new animal model and media to isolate and grow OPCs. The brains of newly hatched chicks of Japanese quails were used to isolate neuronal stem cells. These cells were then grown in media comprising of quail's egg yolk and glucose. After two weeks of growth, OPCs were isolated from mixed neuronal cell culture using orbital shaker after overnight centrifugation. OPCs separate from the mixture and were isolated for further growth. Isolated OPCs were then grown using different combinations of egg yolk and glucose. These cells converted into mature myelinating oligodendrocytes after two weeks of growth. Our findings confirm the successful application of natural media for OPCs isolation and growth which can potentially contribute towards future therapeutic approaches in neuroscience.

Publication Details

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DOI DOI not available

Published Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022

Keywords

Neuroscience Neonatal quail brain Oligodendrocytes

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