Crop Traits Improvement through Genetic Modification and Genome Editing Approaches | [Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 • 2023]

Author(s):

1. Abdul Qayyum Rao: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

2. Allah Bakhsh: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

3. Ayesha Latif: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

4. Saira Azam: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

5. Aneela Yasmeen: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

6. Naila Shahid: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

7. Ambreen Gul: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

8. Sana Shakoor: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

9. Sahar Sadaqat: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

10. Sara Ajmal: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

11. Muhammad Awais: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

12. Muhammad SaadBhutta: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

13. Ramsha Ajmal: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

14. Muhammad Saad Tahir: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

15. Maham: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

16. Hafiz Muhammad Zeeshan: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

17. Sania Naeem: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

18. SamaviaWirk: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

19. Muntaha Khalid: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

20. Samran Sardar: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

21. Tahir Rehman Samiullah: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

22. Munim Farooq: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

23. M. Tariq: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

24. M. Usman Arif: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

25. Farah Naz: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

26. Saman Riaz: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

27. Rida Hameed: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

28. Saqib Ali: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

29. Noman Nazik: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

30. Zil Huma: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

31. Muneeba Shahid: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

32. Atif Shahzad: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

33. Momna Muhammad: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

34. Mehwish Imtiaz: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

35. Ujala Nasir: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

36. Kausar Malik: CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan

Abstract:

CEMB, being pioneer in developing insect and weedicide resistant crops, is successful in approval of its insect and weedicide resistant cotton varieties on commercial basis. To further enhance the toxin level next generation Btalong with fusion gene strategy is also addressed to cope with challenges of resistance buildup in insect pests. Advancement of technology to edit different traits of crop plant is fundamental to be utilized for improvement of major traits of plants like dealing with challenge of CLCuV in cotton, enhancing the storage life of potato, development of potato with enhanced vitamin A and development of resistance in potato against fungus and PVX, PVY. Further abiotic stress tolerant crop is the challenge of current and years to come to be dealt with genome editing is also established at CEMB. Two success stories of genome editing through CRISPR Cas9 system includes knock out of CLCuV targeting DNA A and Beta satellite akong with improved shelf life of potato through knockout of Vlnv has been discussed in current presentation. The knockout efficacy of the 72%for DNA-A, 90% of betasatellite was achieved during this study. The qPCR confirmed the successful reduction of the vial titre and the feeding assay showed 90% of mortality in the whiteflies. The study results were also validated by the change in Alpha chain, Beta Chains, and loops of the 3D protein models of native and edited proteins predicted with Alpha Fold2.The knockout efficiency and the virus inoculation assay magnificently determined the faith in using this technology for plant virus control along with its vector. The amplicon sequencing data showed maximum indel frequency for potato plant T12 (14.3%) resulting in 6.2%gene knock out and 6% frame shift. Whilefor plant B4, the maximum indel frequency of 2.0% was found which resulted in 4.4%knockout and 4%frameshift asanalyzed by Geneious. The qRTPCR data revealed that mRNA expression of VInvgenewasreduced90-99-fold in edited potato plants when compared to the non-edited control potato plant. Following coldstorage, chipsanalysis of potato esproved B4 and T12 asbest lines. Reducing sugars' analysis by titration method determined five fold reduction in percentage of reducing sugars in tubers of B4 transgenic lines as compared to the control.

Page(s): 6-6

DOI: DOI not available

Published: Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords:

genetic modification , Genome Editing Approaches , Crop Traits

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