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Vitamin C inhibits LPS-induced secretion of inflammatory cytokines in peripheral blood
Author(s):
1. Xu-qing Liu: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
2. Qiu-mei Lin: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
3. Chen-wei Zhang: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
4. Han-ling Zhang: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
5. Ming-jie Li: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
6. Rui-ying Li: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
7. Size Chen: Department of Immuno-Oncology, The First Affiliated Hospital of Guangdong Pharmaceutical niversity, Guangzhou, China;Guangdong Provincial Engineering Research Center for Esophageal Cancer PrecisionTherapyGuangdong PharmaceuticalUniversity, Guangzhou, China;Key Laboratory of Cancer Immunotherapy of Guangdong High Education Institutes, Guangdong Pharmaceutical University,Guangzhou, China;Guangdong Provincial Key Laboratory for Monitoring of Adverse Effects Associated with CAR-T Cell Therapies,Guangdong Pharmaceutical University, Guangzhou, China
8. Yu-bin Guan: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
9. Shuo-qi Jiang: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
10. Jia-yi Wu: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
11. Yi-qi Lin: The First Affiliated Hospital/The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University,Guangzhou,PR China
12. Ya-wen Yang: Department of Immuno-Oncology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China
13. Zhao-shou Yang: Department of Immuno-Oncology, The First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou,China
Abstract:
Despite extensive evidence demonstrating that vitamin C suppresses the secretion of inflammatory cytokines, research exploring its impact on cytokine release from peripheral blood cells upon bacterial antigen stimulation remains limited. This research investigated the influence of vitamin C on LPS-triggered secretion of cellular inflammatory cytokines in peripheral blood. Ten healthy volunteers provided heparinized whole blood, diluted with RPMI-1640, and distributed into containers. Cytokines (IL-10, IFN-?, IL-4, TNF-a, IL-2, and IL-6) were quantified using flow cytometric cytokine detection kits. Results showed that LPS significantly increased IL-10 (baseline 2.7±1.35 pg/mL to 34.1±14.86 pg/mL), TNF-a (1.5±0.71 pg/mL to 1136.4±476.56 pg/mL) and IL-6 (1.8±0.59 pg/mL to 2033.4±755.29 pg/mL) levels, while IFN-?, IL-4 and IL-2 remained unaffected. Vitamin C treatment significantly inhibited the secretion of IL-10 (23.5±5.5 pg/mL, P=0.0019), TNF-a (407.3±96.44 pg/mL, P=0.0389) and IL-6 (1572.4±444.43 pg/mL, P=0.0275). Both LPS and vitamin C caused a mild reduction in red blood cell counts, but no hemolysis was observed. These findings highlight vitamin C's potential to inhibit LPS-induced inflammation in peripheral blood, providing a basis for its therapeutic application in bacterial infection-related inflammatory diseases.
Page(s): 249-259
Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 38, Issue: 1, Year: 2025
Keywords:
Vitamin C , Peripheral blood , inflammatory cytokines , LPS
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