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Biochemical, Phytochemical, and biological evaluation of Nymphaea lotus from Cholistan Desert
Author(s):
1. Amna Irshad: Department of Biochemistry, IBBB, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
2. Mirza Imran Shahzad: Department of Biochemistry, IBBB, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Abstract:
Herbal medicine has been in practice since long ago but recently it has gained the valuable fraction of medicine industry due their ease to use, social acceptance, and cost effectiveness. Their effective use against drug resistant strains of microbes is another reason of their gain in popularity. The aim of this study was Biochemical, Phytochemical, and Biological evaluation of different parts of Nymphaea alba Lotus through different extracts. Leaves and rhizome extracts were screened for their phytochemical analysis, antibacterial, antiviral, and antioxidant potentials. The results showed significant levels of antibacterial, antiviral and antioxidant activities from these extracts. Disc diffusion method was used for antibacterial activity. Antibiofilm potential was checked in round bottom 96-well plates by using crystal violet dye and taking OD at 630nm. Rhizome extracts have shown greater antibacterial potential as compared to leaves. To evaluate the antiviral potential of the extracts, Hemagglutination test (HA) was performed against AIV H9N2 and IBV strains. Ethanol and n-hexane extracts of leaves while Methanol, n-hexane, and Aqueous extracts of rhizome were found active against AIV H9N2. Ethanol and n-hexane extracts of leaves while Ethanol and Aqueous extracts of rhizome were found active against IBV. The antioxidant potential was measured by FRAP and DPPH assays. Methanol, Ethanol, and Ethyl acetate extracts of leaves and rhizome were found equally good in antioxidant potential. Qualitative tests were performed to screen the phytochemical composition which revealed that this plant is rich in flavonoids but other phytochemicals like carbohydrates, protein, lipids, saponins, tannins, phenols, and glycosides are also found in varying concentrations. The study concludes that the desert plant N. alba L is rich source of pharmacological and biological agents and their extracts can be used in both crude and purified form
Page(s): 97-97
DOI: DOI not available
Published: Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Cholistan desert , biological evaluation , Nymphaea lotus
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