The coenocytic filaments of two green algae, Cladophora glomerata (Linnaeus) KÜtzing and C.okamurai (Ueda) van den Hoek from freshwater habitats of Sindh Province of Pakistan and extracted in methanol. Their crude extracts showed strong antimicrobial activity against 14 bacterial and 20 fungal species including 7 human-, 5 plant- pathogens and 8 saprophytes, but their cytotoxic activity against brine shrimp larvae was non-significant. Methanol extracts of the two species indicated the presence of 38 different fatty acids including 15 saturated and 23 unsaturated acids, while acids of both the categories were in almost equal proportion (46.5-51.9 % saturated, 48.1-53.5 % unsaturated). Furthermore 4 sterols, 4 terpenes, 2 carbohydrates and 1 glycoside were also obtained from these extracts. Both the species differed in the composition of their natural products.