Abstract:
Apart from typical natural processes, excessive industries and urbanization release significant amounts of dyes into water bodies, which is the primary source of environmental contamination. Even in minute amount (less than 1mg/L for some dyes), their presence is extremely unwanted and objectionable. Here the aim of this study was to make a new solvent system based upon on the method of liquid - liquid microextraction method for the pre concentration of cationic and anionic dyes in water sample prior to UV-visible spectrophotometry. The pH effect, volume of sample, volume of supramolecular solvent (SS) and vortex time were all optimized to enhance the method extraction efficiency. A 50 mL centrifuge tube was used to hold the dye sample solution. Phosphate buffer solution of required pH was added to the dye solution. After pH adjustment, supramolecular solvent (decanol + THF) were added to the sample solution and subjected to vortex for three minutes to ensure complete mixing of the solution. To complete the phase separation, the solution was then centrifuged at 4000 rpm for 10 min. The extracted phase was separated and then diluted with ethanol. The Rhodamine B and EBT dye were extracted quantitatively into the supramolecular solvent (SS) phase and measured spectrophotometrically at 550nm and 530nm respectively. The optimized pH for Rhodamine B and EBT were pH=6 and pH=3, initial concentration of dye 10ppm and 10ppm, volume of sample 10ml and 10ml, volume of supramolecular solvent 0.9ml and 0.9ml, vortex time 7min and 5min respectively. We will further analyse tap water, sea water and dam water (Tanda Dam Kohat) using addition recovery tests for water samples to evaluate the accuracy of the suggested method.
Page(s):
74-74
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Life Sciences (ICLS-23) 11-12 May 22-23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Preconcetration
,
Cationic and Anionic Dyes
,
Supramolecular SolventBased LiquidLiquid Microextraction