ZOO-1523: Herbal Modulation of Growth and Immune Responses in Labeo rohita Against Aeromonas hydrophila | [4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025 • 2025]

Author(s):

1. Aiman Nadeem: Department of Zoology, Government College University Faisalabad, Pakistan.

2. Farkhanda Asad: Department of Zoology, Government College University Faisalabad, Pakistan.

3. Rafia Jamal: Department of Zoology, Government College University Faisalabad, Pakistan.

4. Rida Nadeem: Department of Zoology, Government College University Faisalabad, Pakistan.

5. Maimoona Mehmood: Department of Zoology, Government College University Faisalabad, Pakistan.

6. Maryam Fatima: Department of Zoology, Government College University Faisalabad, Pakistan.

7. Itrat Fatima: Department of Zoology, Government College University Faisalabad, Pakistan.

8. Saba Naseer: Department of Zoology, Government College University Faisalabad, Pakistan.

Abstract:

An experimental study was conducted to evaluate the effects of Achyranthesaspera and Ricinus communis seed extracts on the growth performance and immune response of Labeo rohita. One hundred twenty fish (initial weight6.50±0.004g) were purchased from local hatchery in Punjab and acclimatized under laboratory conditions on a control diet for one week. Ethanol extracts of plant seeds were prepared and incorporated into the basal diet. The fish were randomly assigned to six aquaria (20 fish per aquarium) with replicates and fedat 4% of their body weight twice daily for 60 days. Treatments included a control group (T0) and two experimental groups: T1: MP1 (basal diet + 2 g A. asperaseed extract) and T2:MP2 (basal diet + 2 g R. communis seed extract). Water quality parameters were maintained throughout the trial. The results showed that fish in T1 group exhibited the highest growth rate and weight gain (p < 0.05),followed by fish in the T2 group. Both T1 and T2 groups demonstrated significantly elevated serum IgM levels compared to the control (p < 0.05). A.hydrophila was cultured in tryptic soy broth (TSB) to prepare the bacterial challenge inoculum used for assessing disease resistance in L. rohita. After 60days, ten fish from each group were challenged with live A. hydrophila andobserved for 10 days. Cumulative survival was highest in T1, intermediate inT2, and lowest in the control. The control group also displayed the highest mortality rate with evident clinical signs of bacterial infection. These findings indicate that seed extracts of A. aspera and R. communis enhance growth and immune response in L. rohita and may serve as natural alternatives toantibiotics. It is concluded that these herbal extracts are effective for treating fish diseases, reducing drug resistance, and minimizing residual drug accumulation in fish and consumers.

Page(s): 197-197

DOI: DOI not available

Published: Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025

Keywords:

immunity , Growth , Labeo rohita , Achyranthes aspera , Ricinus communis

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