Analysis of SNP of Kisspeptin Gene in Diabetes Mellitus Type 2 Patients in District Gujrat, Punjab | First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book) 2022

Author(s)

Israr-ud-Din University of Gujrat,Hafiz Hayat Campus, Gujrat,Pakistan

Shazia Shamas The Rawalpindi Women University,Rawalpindi,Pakistan

Areej Farooq University of Gujrat,Hafiz Hayat Campus, Gujrat,Pakistan

Rabea Ejaz The Rawalpindi Women University,Rawalpindi,Pakistan

Sadia Roshan University of Gujrat,Hafiz Hayat Campus, Gujrat,Pakistan

Shagufta Nighat The Rawalpindi Women University,Rawalpindi,Pakistan

Abstract

-Type 2 diabetes mellitus is a major metabolic disorder in which the glucose level of an individual increases from the normal level due to low level of insulin production. Kisspeptin gene forming a peptide hormone which play a key reproductive, metabolic role and any abnormality in KISS1 gene can leads to reproductive (PCOS, CPP) and metabolic disorders (diabetes, obesity). The objective of the current study was to identify and examine the association of KISS1 nsSNP (rs1303540530) with T2DM in District Gujrat, Pakistan. Initially different bioinformatics tools were used for the identification of deleterious nsSNPs of kisspeptin. A single KISS1 nsSNP (rs1303540530) was selected out of 301 nsSNPs and against this nsSNP primer was designed. After that for the experimental work 70 (35 T2DM and 35 control group) blood samples were taken. The DNA was extracted from the whole blood through phenol chloroform method and the ARMS-PCR was used for the amplification of DNA and detection of KISS1 nsSNP (rs1303540530) through designed primers. In our study no double band were seen in all T2DM and control group. Thus, the nsSNP (rs1303540530) were not identified and showed no association with T2DM. The demographic study revealed that age, body mass index (BMI) and blood group show significant association while gender show no association with T2DM. Further studies in larger population or people from different regions are needed to verify and explore our findings.

Publication Details

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DOI DOI not available

Published Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022

Keywords

SNPs Diabetes Mellitus Kisspeptin

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