Abstract:
Acid and alkaline phosphatases were quantitatively determined by the procedure described earlier with p- nitrophenolas the colorimetric standard and disodium p-nitrophenol phosphate as the substrate . The pH optima of acid and alkaline phosphatases were found to vary at different stages, thus indicating the effect of age on the optimum pH of the enzymes. In the case of acid phosphatases the pH optima were recorded as 4-7 for the egg, 4.6 for the 1st instar hopper, 4.5 for the 2nd and 3rd instar hopper and 4.4 for the 4th and 5th instar hopper and adults. In the case of the alkaline phosphatase the pH optima were determined as 7.8 for the egg, 7.5 for the 1st and 2nd instar hopper and 7.4 for the 3rd, 4th, and 5th instar hopper and adults.
Page(s):
607-616
DOI:
DOI not available
Published:
Journal: Science & Industry, Volume: 5, Issue: 4, Year: 1967