Abstract:
To assess the frequency and degree o f pituitary hyperplasia in albino rats made hypothyroidby Carbimazole, and the association o f severity of pituitary enlargement, and the response to treatment withthroxine. To determine the microscopic changes occurring in Beta basophil cells (Thyroid stimulatinghormone & Adreno corticotrophin hormone producing cells) o f Anterior Pituitary gland by giving an antithyroiddrug, Carbimazole and Carbimazol plus Thyroxin on Anterior pituitary glands o f male albino ratswith increasing time period.Design' Experimental studyPlace: Anatomy Department, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre Karachi.Methods: Forty five healthy, young adult male albino rats were selected for the study. They were distributedinto 3 main groups o f 15 rats each. Group A served as control while Group B received injection Carbimazole6 ^gm/G body weight subcutaneously daily. Group-C were treated with injection Carbimazole 6 ^gm/Gbody weight subcutaneously plus injection Thyroxin 5 ^gm intraperitoneally daily for their respective periodo f treatment. Each group was further subdivided into three sub-groups according to the period o f treatmentthey received i.e. 2, 4 & 6 weeks at the end o f which animals were sacrificed. The Pituitary glands weredissected out after processing and staining (Wilson-Ezrin method). The tissues were subjected to detailedmicrometric examination.Result' The results are based on changes in morphometric study o f number and diameter ofBeta Basophilcells in anterior Pituitary gland. Mean value o f number o f Basophils were increased significantly (P <0.001) in group B (Carbimazole treated) i.e 151.0± 3.38 than group A (control) i.e 82.2 ± 3.48. While in group C (Carbimazole plus Thyroxin treated) the number of cells were decreased i.e 117.6 ± 3.83 than group B but were more than group A, Beta Basophil cell size (diameter) was also increased significantly (P <0.001) in group B i.e 17.64 ± 1.06 than group A 14.45 ± 3.28. In group C Beta Basophil cell size was16.16 ± 2.02, which was more than group Abut less than group C. The number and size o f Beta Basophils in group C was significant (P<0.05), when compared with corresponding controls. Conclusion'. In conclusion these results strongly suggest that Carbimazole-induced hyperplasia and hypertrophy o f Anterior pituitary gland may be prevented by simultaneous treatment with Thyroxin. Long standing treatment with Carbimazole in Hyperthyroid patients as in Graves disease should accompany, small doses o f Thyroxin as well, to avoid the enlargement o f Anterior Pituitary gland during their treatment.
Page(s):
49-54
DOI:
DOI not available
Published:
Journal: Journal of Dow University of Health Sciences, Volume: 3, Issue: 2, Year: 2009
Keywords:
Adenohypophysis
,
pituitary
,
Thyroxin
,
Hyperplasia
,
Carbimazole