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The Bacterial Load and Contamination Rate of Raw Meat in Butcher Shops in Different Areas of Karbala City
Author(s):
1. MUSTAFA ABDUL KAREEM HAMEED: Department of Veterinary Microbiology, College of Veterinary Medicine, University of Karbala,,Iraq
2. HAYDER ALI MUHAMMED: Department of Veterinary Microbiology, College of Veterinary Medicine, University of Karbala,,Iraq
3. ASHRAF AYYAL MUTAR ALRASHEDI: Department Medical Laboratory Technical, Alsafwa University College,Karbala,Iraq
Abstract:
This study was aimed at assessing the types of bacterial isolates and their antimicrobial agentsprofile identified from raw meat and meat products butcher shops indifferent areas of Karbala city,A total of 100 random samples from raw meat were purchased from different butcher shops in Karbala city between October 2019 to February 2020 for the isolation and identification of microbial pathogens, The antibiotic resistant characteristics of the isolated organisms was determined by using Kirby-Bauer disk diffusion method and Polymerase chain reaction assaywas used to confirmall bacterial isolates obtained from direct colony of 24 hour by using16S rRNA genes, the results showed Escherichia coli was most Gram-negative found on meat samples about(45 samples) with percentage 45% fromall samples, 30% from samples contaminated with Gram-positivebacteria (Staphylococcus) and 6% was recorded as Salmonella, on the other hand , The results showed that the Ciprofloxacin and Gentamicin were susceptible to 95% of all isolates.
Page(s): 690-692
DOI: DOI not available
Published: Journal: Pakistan Journal of Medical and Health Sciences, Volume: 15, Issue: 3, Year: 2021
Keywords:
PCR , Ecoli , Salmonella , Staphylococcus , raw meats
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