Author(s):
1. Mona Mohamed Amin:
Health Radiation Research Department, National Centre for Radiation Research and Technology (NCRRT) Nasr City,Egypt
2. Eman Naser Hafez:
Health Radiation Research Department, National Centre for Radiation Research and Technology (NCRRT) Nasr City,Egypt
Abstract:
Toxoplasma gondii (T. g) infection causes severe lethal disease on fetus and recipients. The study was designed to study the ultrastructure of 300Gy attenuate irradiated tachyzoite and histopathological alterations induced in vaccinated host. The alterations in T. gtachyzoite irradiated with attenuating dose of gamma radiation using Transmission Electron Microscopy (TEM). The histopathological changes produced in spleen, lung, kidney and testis in mice infected with 2x103 RH virulent tachyzoites and others vaccinated with 300 Gy gamma-irradiated tachyzoites and challenged at 21st day with 2x103 with highly virulent RH T. gondii tachyzoites were evaluated. Transmission electron microscopy revealed a uniform size, a smooth surface and intact cell or nuclear membranes with an ovalshaped nucleus also conoids and micronemes were observedin control group. By contrast, gamma-irradiated tachyzoites group showed vacuolization in their cytoplasm with the substantial reduction in the number of dense granules and the blur of some organelles. On the other hand, infected groups revealed massive histopathological changes which were markedly decreased in vaccinated group.
Page(s):
1325-1333
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Zoology, Volume: 47, Issue: 5, Year: 2015
Keywords:
histopathology
,
Toxoplasma gondii
,
radiation
,
TEM
,
gamma
References:
References are not available for this document.
Citations
Citations are not available for this document.