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The expression of visfatin in mouse ovary and its regulatory effect on ifn-?.
Author(s):
1. Hui Song: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
2. Qiu-xia Lin: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
3. Rui-an Li: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
4. Ke Xiao: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
5. Ke-mei Peng: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Abstract:
The expression of visfatin in ovary and the changes of IFN-γ in peripheral blood were detected by the immunohistochemical method and the double antibody sandwich ELISA test method to investigate the distribution situation of visfatin in the ovary and its regulatory effect on the development of mouse ovarian follicles. Our research showed that visfatin positive cells were mainly located in the follicle cell layer of ovary in different developmental stages. The strongly positive staining were seen in the cells of the granular layer of growing follicle, the positive cells were numerous and compressed, and the cells of the granular layer of mature follicle also showed significantly positive reaction though their amount was fewer. Visfatin positive cells were rarely expressed and were weakly positive in oocytes, and it lacked in germinal epithelium, theca folliculi, follicular matrix and atretic follicle. Statistical results showed that the visfatin level of the control group was not significantly different from that of the protein group or the antibody group in the peripheral blood, while the protein group was significantly different from antibody group (P
Page(s): 180-184
DOI: DOI not available
Published: Journal: Pakistan Veterinary Journal, Volume: 34, Issue: 2, Year: 2014
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