A highly efficient regeneration system for watermelon (Citrullus lanatus Thunb.). | [Pakistan Journal of Botany • 2013]

Author(s):

1. Xuezheng Wang: Horticulture College, Northeast Agricultural University, Harbin 150030, P.R. China

2. Limin Shang: Horticulture College, Northeast Agricultural University, Harbin 150030, P.R. China

3. Feishi Luan: Horticulture College, Northeast Agricultural University, Harbin 150030, P.R. China

Abstract:

We developed a high frequency watermelon regeneration system using two inbred lines of watermelon (Citrullus lanatus), ‘W1-4’ and ‘W1-12’. Shoots were induced from cotyledonary nodes cultured on Murashige and Skoog (MS) basal medium solidified with agar (7.0 g/L) and containing various concentrations of cytokinin (6-benzyladenine) 6-BA) and auxin (indoleacetic acid) IAA). The highest rate of bud organogenesis was on MS medium containing 1.5 mg/L 6-BA + 0.2 mg/L IAA for ‘W1-4’ and on MS medium containing 1.0 mg/L 6-BA + 0.1 mg/L IAA for ‘W1-12’. The regeneration rate was higher in ‘W1-12’ than in ‘W1-4’. The best medium for shoot elongation in both inbred lines was MS containing 0.05 mg/L 6-BA. Regenerated plants showed the best rates of root formation on 1/2 MS containing 0.1 mg/L IAA. The rooted plants were carefully washed to remove all medium from the roots, and then transferred to soil in a greenhouse. The plants showed a 100% survival rate when transferred to soil. This highly efficient regeneration system will be useful for regenerating plants in genetic engineering applications, and is a useful tool for further genetic transformation studies on watermelon.

Page(s): 145-150

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 45, Issue: 1, Year: 2013

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