Taxonomic significance of pollen, anatomical and morphological characteristics of red and green leaves christia vespertilionis (L.F) bakh. F. (fabaceae) | [Pakistan Journal of Botany • 2024]

Author(s):

1. NORAINI TALIP: Department of Biological Sciences and Biotechnology, Bangi Botanic Gardens, Faculty of Science and Technology, Universiti Kebangsaan Malaysia,43600 Bangi, Selangor,Malaysia

2. CHE NURUL AINI CHE AMRI: Department of Plant Science, Kuliyyah of Science, International Islamic University Malaysia,Kuantan Campus, 25200 Kuantan,Malaysia

3. MOHAMAD RUZI ABDUL RAHMAN: Department of Biological Sciences and Biotechnology, Bangi Botanic Gardens, Faculty of Science and Technology, Universiti Kebangsaan Malaysia,43600 Bangi, Selangor,Malaysia

4. MUHAMMAD AMIRUL AIMAN AHMAD JUHARI: Department of Environment, Faculty of Forestry and Environment, Universiti Putra Malaysia,43400 Serdang,Malaysia

5. MOHD. NORFAIZAL GHAZALLI: Programme of Resource Utilisation and Agrobiodiversity Conservation, Agrobiodiversity & Environment Research Centre,43400 Serdang,Malaysia

6. AHMAD FITRI ZOHARI: Department of Biological Sciences and Biotechnology, Bangi Botanic Gardens, Faculty of Science and Technology, Universiti Kebangsaan Malaysia,43600 Bangi, Selangor,Malaysia

7. HAMIDUN BUNAWAN: Institute of Systems Biology, Universiti Kebangsaan Malaysia,Bangi 43600, Selangor,Malaysia

Abstract:

Anatomy, morphology and palynology studies were conducted on green and red Christia vespertilionis (L.f.) Bakh.f. The objective of this study is to identify the common, variation and diagnostic features and to construct key identification of species. Anatomy study involves sectioning using sliding microtome on the petiole, lamina, midrib and leaf margin, epidermal mechanical scrapping to observe epidermis, leaf clearing for type of venation, for observation under light microscope, images are captured using Olympus DP25 camera and images analyzed using Docu Analysis software. Leaf and flower morphological features are measured for morphology study. Pollen study involves sample preparation, acetolysis techniques and observation under a light microscope. Common features obtained from this study such are the presence of crystals and muscilaginous cells, presence of additional vascular bundles in the petiole and midrib, the presence of air spaces between the cells, pattern of anticlinal cells wall, presence of glandular and papillae trichomes, number of leaf veins, pedicel characteristics, number of petals, pollen class, aperture type, pollen size, and exine pattern. Variation than can be used to distinguish green and red leaf C. vespertilionis are outline shape of leaf lamina, presence of swollen tracheid, type of trichome, vascular tissue arrangement in the midrib and petiole, presence and type of stomata, leaf colour, petiole characteristics, terminal leaf characteristics, abaxial and adaxial leaf surface, main leaf and calyx feature, pollen shape and exine thickness. The results have shown high variation between green and red C. vespertilionis. In conclusion, this study proves that green and red leafy C. vespertilionis are likely different species, subspecies or varieties and accordingly a status study should be done on both green and red leafy to see their taxonomic position.

Page(s): 557-565

DOI: 10.30848/PJB2024-2(37)

Published: Journal: Pakistan Journal of Botany, Volume: 56, Issue: 2, Year: 2024

Keywords:

anatomy , Morphology , anatomy , Palynology , Christia vespertilionis , Rerama

References:

[1] Allen O.N.,Ethel K.A. .1981 .The Leguminosae. , : .

[2] Anonymous .2017 .The International Plant National Index. html [18 April, : .

[3] Anonymous .2012 .Christia vespertilionis. , : .

[4] Anonymous .2017 .Christia vespertilionis. aspx?id=1811 [25 Oct, : .

[5] Badron T.,Noraini A.L.,Mohamad A.E.A.,Affenddi A.A.A.,Juhari A.A.A. .2014 .Studies on leaf venation in selected taxa of the genus Ficus l. (Moraceae) in Peninsular Malaysia. Trop. Life Sci. Res, 25(2) : 111-125.

[6] Barham J.M. .1996 .Christia vespertilionis var. vespertilionis. Curtis's Bot. Mag., 13(1) : 19-21.

[7] Chung R.C.K.,Soepadmo E.,Lim A.L. .2003 .The significance of pollen morphology in the taxonomy of Grewia and Microcos (Tiliaceae) in Peninsular Malaysia and Borneo. Gard. Bull.Sing, 55 : 239-256.

[8] Croteau R. .1977 .Site of monopertene biosynthesis in Majorana hortensis leaves. Plant, 59 : 519-520.

[9] Cutler D.F.,Brotha C.E.J.,Stevenson D.W. .2008 .Plant Anatomy: An Applied Approach. , : .

[10] Dash G.K.,Bakh G.K. .2016 .A promising medicinal plant. Int. J. Pharmacogn. Phytochem. Res., 8(6) : 1037-1039.

[11] Dickison W.C. .2000 .Integrative Plant Anatomy. , 1 : .

[12] Dilcher D.L. .1974 .Approaches to the identification of angiosperm leaf remains. Bot. Rev., 40(1) : 1-157.

[13] Duke S.O. .1994 .Commentary on glandular trichomes-A focal point of chemical and structural interactions. Int. J. Plant Sci., 155 : 617-620.

[14] Erdtman G. .1952 .Pollen Morphology and Plant TaxonomyAngiosperm. , : .

[15] Erdtman G. .1969 .Handbook of Palynology. An Introduction to the Study of Pollen Grains and Spores. , : .

[16] Firoze-Quamar S. Nawaz,Ali P.,Morthekai V.K.,Singh V.K. .2017 .Confocal (CLSM) and light (LM) photomicrographs of different plant pollen taxa from Lucknow, India: Implications of pollen morphology for systematics, phylogeny and preservation. Rev. Palaeobot. Palynol., 247 : 105-119.

[17] Franceschi V.R.,Jr H.T. .1980 .Calcium oxalate crystals in plants. Bot. Rev., 46 : 361-361.

[18] Frenguelli G. .2004 .Pollen structure and morphology. Advances in dermatology and allergology/postępy dermato\logii i. Alergologii, 20(4) : 200-204.

[19] Hamidun B.,Siti-Noraini B.,Nataqain B. Syarul .2015 .The red butterfly wing (Christia vespertilionis): A promising cancer cure in Malaysia. Int. J. Pharm. Pharm. Sci., 8(11) : 2219.

[20] Metro Harian .2016 .Tea Rerama Intellectual Property. , : .

[21] Hewson H.J. .1988 .Plant indumentum. A Handbook of Terminology. , : .

[22] Hofer D.,Schwach G.,Tabrizi-Wizsy N.G.,Sadjak A.,Sturm S.,Stuppner H.,Pfragner R. .2013 .Christia vespertilionis plant extracts as novel antiproliferative agent against human neuroendocrine tumor cells. Oncol. Rep, 29(6) : 2219-2226.

[23] Huang P.,Hiroyashi O.,Moench Christia,Z.Y. Christia,Raven P.H.,Hong D.Y. .2010 .10th edition. , 129 : 289-290.

[24] Johansen D.A.,Microtechnique. McGraw-Hill Co Plant .1940 .. , : .

[25] Langran X.,Dezhao C.,Xiangyun Z.,Puhua H.,Zhi W.,Ren S.,Dianxiang Z.,Yingxin Y.,Zhaoyang C.,Jianqiang L.,Mingli Z.,Z.Y. Z.,Raven P.H.,Hong D.Y. .2010 .10th edition. , : 1-577.

[26] Maideen H.,Hazwani A.N.,Nurfarahain Z.,Damanhuri A.,Noraini T.,Rusea G.,Qistina L.,Masnoryante M. .2013 .Systematic significance of stipe anatomy of Selaginella (Selaginellaceae) in Peninsular Malaysia. , 42(5) : 693-696.

[27] Marcati C.R.,Angyalossy V. .2005 .Seasonal presence of acicular calcium oxalate crystals in the cambium zone of Citharexylum myrianthum (Verbenaceae). IAWA J, 26 : 93-98.

[28] Metcalfe C.R.,Chalk L. .1950 .Anatomy of the Dicotyledons. , 1 : .

[29] Metcalfe C.R.,Chalk L. .1957 .Anatomy of the Dicotyledons. , 2 : .

[30] Metcalfe C.R.,Chalk L. .1979 .Anatomy of the Dicotyledons. , : .

[31] Nguyen-Pouplin J.H.,Tran H.,Tran T.A.,Phan C.,Dolecek J.,Farrar T.H.,Tran P.,Caron B.,Bodo P.,Grellier P. .2007 .Antimalarial and cytotoxic activities of ethnopharmacologically selected medicinal plants from South Vietnam. , 109(3) : 417-427.

[32] Noraini T,Hussin K.H.,Ibrahim H. .2003 .Comparative leaf anatomy of Alpinia species (Zingiberaceae) in Malaysia. , 23(4) : 463-483.

[33] Noraini T.,Cutler D.F. .2009 .Leaf anatomical and micromorphological characters of some Malaysian Parashorea (Dipterocarpaceae). J. Trop. For. Sci., 21(2) : 156-167.

[34] Noraini T.,Amirul-Aiman A.J.,Jaman R.,Damanhuri A.,Ruzi A.R. .2014 .Systematic significance of stipe anatomy in peninsular Malaysian Blechnum L. (Blechnaceae) species. , 43(2) : 119-128.

[35] Noraini T.,Ruzi A.R.,Ismail B.S.,Salwa S.,Azeyanty J.A. .2016 .Petiole vascular bundles and its taxonomic value in the tribe Dipterocarpeae (Dipterocarpaceae) Sains Malays. , 45(2) : 247-253.

[36] Noraini T.,Ruzi A.R.,Nadiah N.,Maideen K.M.,Solihani S.N. .2012 .Stipe anatomical characteristics in some Davallia (Davalliaceae) species in Malaysia. , 41(1) : 53-62.

[37] Noraini T.,Cutler D.F.,Ahmad Puad A.S.,Ismail B.S.,Ruzi A.R.,Muhammad A.J. .2017 .Diagnostic and systematic significance of petiole anatomy in the identification of Hopea species (Dipterocarpaceae). S. Afr. J. Bot., 111 : 111-125.

[38] Osman M.S.,Ghani Z.A.,Ismail N.F.,Razak N.A.A.,Jaapar J.,Ariff M.A.M. .1885 .Qualitative comparison of active compounds between red and green Mariposa Christia vespertillonis leaves extracts. AIP Conf. Proc., : .

[39] Prychid J.R.,Rudall P.J. .1999 .Calcium oxalate crystals in monocotyledons: A review of their structure and systematics. , 84 : 725-739.

[40] Rajandeep K.,Harpreet K. .2017 .Plant derived antimalarial agents. J. Med, 5(1) : 346-363.

[41] Rudall P. .2007 .Anatomy of Flowering Plants: An Introduction to Structure and Development. , : .

[42] Sass J.E. .1958 .. Ed: 3rd. Oxford & IBH Publishing Co., Calcutta, : .

[43] Syamsurina A.,Mohd A.J.,Noraini T.,Azilah A.W. Nor,Syuhada F.,Jumaat A. .2020 .Comparison of leaf anatomy of Tetrastigma rafflesiae (Miq.) Planchon and Tetrastigma pedunculare (Wall. ex Laws.) Planch. , 49(4) : 721-728.

[44] Werker E. .1993 .Function of essential oil-secreting glandular hairs in aromatic plants of the Lamiaceae. A review. Flavor Frag. , 8 : 249-255.

[45] Worasitikulya S.,Sununta M.,Pitakpong M. .2022 .Growth and anatomical adaptations in response to salinity stress in Cucurbita moschata Duchesne 'Butternut' (Cucurbitaceae). Sains Malays, 51(5) : 1317-1324.

Citations

Downloads

Views