Three bacteriophages SA, SA2 and SNAP can control growth of milk isolated Staphylococcal species. | [Pakistan Journal of Zoology • 2017]

Author(s):

1. Aiza Tahir: Department of Microbiology and Molecular Genetics, University of the Punjab,Quaide-Azam Campus, Lahore,Pakistan

2. Muhammad Asif: Department of Microbiology and Molecular Genetics, University of the Punjab,Quaide-Azam Campus, Lahore,Pakistan

3. Zaigham Abbas: Department of Microbiology and Molecular Genetics, University of the Punjab,Quaide-Azam Campus, Lahore,Pakistan

4. Shafiq ur Rehman: Department of Microbiology and Molecular Genetics, University of the Punjab,Quaide-Azam Campus, Lahore,Pakistan

Abstract:

Staphylococcus aureus along with other coagulase negative Staphylococci are among the major mastitis causing organisms. Consumers of milk and milk products from mastitic animals are at high risk of foodborne infections. Escalating antibiotic resistance strived us to explore the alternate option to control mastitis. In ongoing era, bacteriophages are an alternate possible effective remedy. Therefore, in this study the infection ability of three lytic phages SA, SANF and SA2 was determined against ten isolates of Staphylococci and one Micrococcus, isolated from five raw milk samples. Their morphological, biochemical characterization and 16S rRNA sequencing indicated that two strains were S. aureus, while all others were different coagulase negative Staphylococci. Their sensitivity against three phages indicated broad host range of phages SANF and SA2, and relatively narrow host range of phage SA. Phage SNAF showed an effective growth reduction of S. aureus RP isolate, compared with other bacteriophages. The bacterial challenge test in milk indicated that proliferation of S. aureus was successfully ceased until six hours post infection when applied at and MOI of 100.

Page(s): 529-533

DOI: 10.17582/journal.pjz/2017.49.2.529.533

Published: Journal: Pakistan Journal of Zoology, Volume: 49, Issue: 2, Year: 2017

Keywords:

Mastitis , Milk , SNAF , bacteriophages

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views