Abstract:
Herbal medicines are considered safe, easy to use and cost effective as compared to allopathic medicines. Their antibacterial potentials against multiple drug resistant strains, is another reason of common use. The current study was designed with aim to screen cholistani plant Caralluma edulis, commonly known as Seetu for its In Vitro (phytochemical, antibacterial, antibiofilm, antidiabetic, antiviral, and antioxidant potentials) ans In Vivo studies. MtOH, EtOH, EA, n-hex, and aqueous extracts were made and used for various activities. HPLC-PDA analysis was done to screen the phytochemical composition. MtOH, EtOH and EA extracts were found rich in phytochemicals. Extracts were tested against E. coli, K. pneumonia, P. aerogenosa, P. vulgaris, S. aureus, MDR S. aureus and MDR P. aerogenosa for antibacterial and antibiofilm activities. Different extracts have shown different levels of activities against selected strains. The antioxidant potential was checked by FRAP and DPPH assays. All extracts were employed in different In Vivo trials on the Rodent model for study. Female albino rats were used for this evaluation process and provided with standard lab conditions according to the OECD guidelines. In In Vivo studies anti inflammatory, antipyretic and antidiabetic activities were performed. EA extract had max activity in FRAP and DPPH assay. Antidiabetic potential was checked by a-glucosidase inhibition assay and n-Hex and EA extracts have shown max. %inhibition. The antiviral potentials were checked through Hemagglutination (HA) test and titers were interpreted through log 2 reduction assay. MtOH extract was most active against IBV and similarly, n-hex extract was most effective against AIV-H 9 N 2 virus. IC 50 of each positive extract was calculated. In Acute toxicity test, no mortality was observed up to the dose level of 5g/kg. In antidiabetic activity, all extracts except MtOH showed significant results. DCM extracts showed max. anti-inflammatory potential, which is dose-dependent. The antipyretic potential of C. edulis extracts was investigated using the Yeast-induced pyrexia Model. All extracts showed significant antipyretic potential, which is dose-dependent. Among them, EA extract had max., and n-Hex had a min. antipyretic potential. The strong antibacterial, antibiofilm, antiviral, antidiabetic and antioxidant activities and rich fullness of phytochemical agents have supported the fact that C. edulis is not only a healthy food but also a great source of pharmacological agent(s). The results of In Vivo trials suggested that C. edulis possess strong anti-inflammatory, antipyretic, and antidiabetic potential, which proves that it is not only a vegetable with many health benefits but could also be an alternate source of medicine and may also lead to some exceptional drug discovery with no harmful effects.
Page(s):
57-57
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
antioxidant
,
Antiinflammatory
,
MDR
,
Cholistan
,
C edulis
,
Antidiabetic
,
HPLCPDA
,
anticorona virus
,
antipyretic