The Ameliorative Effects of L-Carnitine against Cisplatin-Induced Gonadal Toxicity in Rats | [Pakistan Veterinary Journal • 2021]

Author(s):

1. Adham Omar Sallam: Department of Pharmacology; Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt

2. Hanan A. Rizk: Department of Pharmacology, National Organization for Drug Control and Research (NODCAR), Egypt

3. Mahmoud Abdelghaffar Emam: Department of Histology; Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt

4. Sabreen Ezzat Fadl: Biochemistry Department, Faculty of Veterinary Medicine, Matrouh University, 51744 Matrouh, Egypt

5. Ehab Yahya Abdelhiee: Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Matrouh University, 51744 Matrouh, Egypt

6. Hanem Khater: Department of Parasitology; Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt

7. Ashraf Elkomy: Department of Pharmacology; Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt

8. Mohamed Aboubakr: Department of Pharmacology; Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt

Abstract:

Cisplatin (CP) is a highly efficient remedy in cancer treatment, but it adversely affects the testicular tissue. This work assessed the ameliorative efficacy of Lcarnitine (LC) against CP induced oxidative stress in rat testis, via investigating testosterone level and tissue oxidative/antioxidant parameters, histological alterations, and immunohistochemical expressions of intermediate filaments (IFs) proteins; vimentin (VIM) and cytokeratin 18 (CK18). Twenty-eight rats were assigned into four groups (7 rats each) as follows; groups I and II received saline and LC (100 mg/kg b.wt.) respectively orally once daily for 30 days; group III were injected with a single dose of CP (7.5 mg/kg, IP), 27 days after starting the experiment. Group IV received both LC and CP. Injection of CP significantly decreased serum testosterone and glutathione reductase and catalase in the testicular tissues and elevated malondialdehyde. Histologically, testes of the CP treated group revealed marked degenerative changes. Also, overexpression of both VIM and CK18 in testicular tissues were recorded. However, the administration of LC with CP restored the biochemical parameters, histological and immunohistochemical pictures towards the normalcy. Accordingly, LC is recommended as a supplement with chemotherapy to ameliorate its oxidative stress. This is the first study investigating the immunohistochemical expressions of IFs proteins, VIM and CK18, following administration of LC as a protective agent against CP induced testicular toxicity in rats.

Page(s): 147-151

DOI: 10.29261/pakvetj/2020.082

Published: Journal: Pakistan Veterinary Journal, Volume: 41, Issue: 1, Year: 2021

Keywords:

testosterone , Malondialdehyde , Immunohistochemistry , Cytokeratin , Vimentin , Apoptotic markers

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