Abstract:
Staphylococcus aureus (S. aureus) is one of the most common pathogens that cause hospital and community acquired infections in the world. The use of molecular typing methods is essential for the determinations of strains origin, their clonal relations, and epidemiological investigations. Objective: The purpose of this study was to align the nucleotide sequence of different genes of two strains. These strains are responsible for clinical infections like skin and myocardial infections. In it, we have determined the prevalence of different antibiotic-resistant S. aureus isolates. Material and Method: Different software of bioinformatics like CLUSTAL W, Oligocalc, and Primer3 was used for pairwise alignment, to analyse nucleotide sequence, and for primer designing respectively. Results: S. aureus resistance to different antibiotics can be checked by amplifying cloned gene on PCR and then run on a gel against a specific marker to easily diagnose it. These isolates are resistant to methicillin (MRSA). Conclusion: From this method, we can easily identify different pathogenic genes and this study can easily diagnose different diseases on the molecular level.
Page(s):
169-169
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Staphylococcus aureus
,
Epidemiological
,
Infections
,
Cloned gene
,
primer