Abstract:
Rhodococcus are gram positive, oxidase negative, catalase positive non spore forming ubiquitous bacteria, they are well known for their adaptability and versatility. The present study was conducted to isolate the Rhodococcus strains and to characterize them on the basis of biochemical fingerprinting. Isolated strains were used to determine their pancreatic lipase inhibitory activity and their effect on the cholesterol level of chicks by in vivo studies. Seventeen Rhodococcus strains were isolated from twenty- five different soil samples. Three different readings were taken for lipase inhibitory activity at different time intervals, 30 minute, 60 minutes and 20 hours respectively. Three strains 11b, MT3 and 1A showed highest lipase inhibitor activity of 32%, 42% and 45% respectively, among others were selected and given to chicks in diet after pathogenicity test, to analyze their effect on chicks’ lipid profile. These strains were identified through 16SrRNA sequencing. The selected strains MT3, 11b and 1A were used for GCMS analysis to analyze the compounds produced by the organisms in fermentation media containing nutrient broth, ammonium sulphate, glycerol, NaCl, yeast extract, peptone and malt extract as carbon, nitrogen, oxygen and sulphur source. Analysis of Variance (ANOVA) was applied to obtain significant results of antilipase activity and its effect on chicks. Significant variation (p=0.05) for lipase inhibitory activity was obtained. In in vivo studies MT3 (W), 1A (S), 1A (W), 11b (S) and 11b (W) reduced the total cholesterol, LDL and increased HDL level as compared to control but there was no any significant variation (p=0.05) among weight of chicks. GCMS analysis showed the production of 10,13 Octadecadiynoic acid, methyl ester and Hexadecanoic acid, ethyl ester, Phenol, 2,4-bis(1,1-dimethylethyl)-, 1-Nonadecene, Tetradecane, Heptadecane, 2,6,10,15-tetramethyl-, 17-Pentatriacontene, 1-Hexadecanol, Acetic acid, trifluoro-, tetradecyl ester and Eicosane as the secondary metabolites secreted by bacterial strains 1A, MT3 and 11b. Identified compounds can be further purified and analyzed separately for their lipase inhibitor activity and an extensive study can also be planned for further investigation on cholesterol level.
Page(s):
5-5
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
metabolites
,
cholesterol
,
Obesity
,
Lipid profile
,
GCMS
,
lipase inhibitor
,
Rhodococcus