Pakistan Science Abstracts
Article details & metrics
No Detail Found!!
Tea polysaccharide ameliorates glucolipotoxicity-induced oxidative damage and apoptosis of RIN-m5F cells through Nrf2/HO-1 pathway
Author(s):
1. Haiyan Liu: College of Huangjiu, Zhejiang Industry Polytechnic College,Shaoxing, Zhejiang,China
2. Jianwei Zhang: College of Health, Zhejiang Industry Polytechnic College,Shaoxing, Zhejiang,China
3. Liangrong Zhu: College of Huangjiu, Zhejiang Industry Polytechnic College,Shaoxing, Zhejiang,China
Abstract:
Glucolipotoxicity (GLTy) could cause the islet ß cell dysfunction in metabolic diseases such as diabetes. Tea polysaccharide (TPS) has the potential to ameliorate islet damage, but its protective effects on GLTY-induced islet ß cell damages and its mechanism remain unclear. The RIN-m5F cell damage model was constructed by GLTy induction. The effects of TPS on cell damage were investigated from apoptosis, oxidative stress, inflammatory response, cell senescence and cell function. MTT, ELISA, immunofluorescence, flow cytometry, WB and kits were used to detect cell viability, insulin secretion level, inflammatory factors, antioxidant enzyme activity, cell apoptosis rate and related protein expression. Si-Nrf2 was used to verify whether TPS can activate Nrf2/HO-1 signaling to play a protective role. TPS can increase the cell viability, the positive rate of EdU, insulin secretion, anti-inflammatory factor level, and the activities of SOD and CAT, reduce apoptosis, proinflammatory factor levels and the expression of p21, p53 and p16 proteins. By activating Nrf2/HO1 pathway, TPS alleviated oxidative stress damage, thus playing a protective role against GLTY-induced cell damage. TPS effectively reduced GLTY-induced RIN-m5F cells damage through activating Nrf2/HO-1 pathway, providing a new idea and potential drug selection for the treatment of diabetes.
Page(s): 1700-1713
Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 38, Issue: 5, Year: 2025
Keywords:
Oxidative stress , inflammation , Nrf2HO1 , Tea polysaccharide , RINm5F cells , glucolipotoxicity , cell senescence
References:
[1] GBD 2021 Diabetes Collaborators .2023 .Global, regional, and national burden of diabetes from 1990 to 2021, with projections of prevalence to 2050: A systematic analysis for the Global Burden of Disease Study 2021. , 402(10397) : 203-234.
[2] Akash MSH,Rehman K,Liaqat A .2018 .Tumor necrosis factor-alpha: Role in development of insulin resistance and pathogenesis of type 2 diabetes mellitus. J. Cell Biochem, 119(1) : 105-110.
[3] Akinola OB .2016 .Sweet old memories: A review of the experimental models of the association between diabetes, senility and dementia. Metab Brain Dis, 31(5) : 1003-1010.
[4] Amirshahrokhi K and Zohouri A .2021 .Carvedilol prevents pancreatic β-cell damage and the development of type 1 diabetes in mice by the inhibition of proinflammatory cytokines, NF-κB, COX-2, iNOS and oxidative stress. , 138 : 155394.
[5] Ashrafizadeh M. .2024 .Cell death mechanisms in human cancers: Molecular pathways, therapy resistance and therapeutic perspective. J. Cancer Biomol. Ther., 1(1) : 17-40.
[6] Bahadoran Z,Mirmiran P,Ghasemi A .2024 .Adipose organ dysfunction and type 2 diabetes: Role of nitric oxide. Biochem. Pharmacol., 221 : 116043.
[7] Cai Y,Liu S,Ge X,Cheng L,Zhang X .2024 .Inhibitory effect of tea flower polysaccharides on oxidative stress and microglial oxidative damage in aging mice by regulating gut microbiota. Food Funct, 15(23) : 11444-11457.
[8] Chen G,Chen R,Chen D,Ye H,Hu B,Zeng X,Liu Z X .2019 .Tea polysaccharides as potential therapeutic options for metabolic diseases. J. Agric. Food Chem, 67(19) : 5350-5360.
[9] Chen G,Yuan Q,Saeeduddin M,Ou S,Zeng X,Ye H X .2016 .Recent advances in tea polysaccharides: Extraction, purification, physicochemical characterization and bioactivities. , 153 : 663-678.
[10] Cheng Y,Xue F,Yu S,Du S,Yang Y .2021 .Subcritical water extraction of natural products. Molecules, 26(13) : 4004.
[11] Darenskaya MA,Kolesnikova LI,Kolesnikov SI .2021 .Oxidative stress: Pathogenetic role in diabetes mellitus and its complications and therapeutic approaches to correction. Bull. Exp. Biol. Med, 171(2) : 179-189.
[12] Ding Y,Zhang ZF,Dai X Q,Li Y .2012 .Myricetin protects against cytokine-induced cell death in RIN-m5f β cells. J. Med, 15(8) : 733-740.
[13] Du LL,Fu QY,Xiang LP,Zheng XQ,Lu JL,Ye JH,Li QS,Polito CA,Liang YR .2016 .Tea polysaccharides and their bioactivities. Molecules, 21(11) : 1449.
[14] Duan C,Wang H,Jiao D,Geng Y,Wu Q,Yan H,Li C H .2022 .Curcumin restrains oxidative stress of after intracerebral hemorrhage in rat by activating the Nrf2/HO-1 pathway. , 13 : 889226.
[15] Eaves Eskandari E,CJ Eskandari E .2022 .Paradoxical roles of caspase-3 in regulating cell survival, proliferation, and tumorigenesis. J. Cell Biol, 221(6) : e202201159.
[16] Facchinetti MM .2020 .Heme-oxygenase-1. , 32(17) : 1239-1242.
[17] Fan Y,Zhou X,Huang G .2022 .Preparation, structure, and properties of tea polysaccharide. Chem. Biol. Drug Des, 99(1) : 75-82.
[18] Guo W,Song Y,Sun Y,Du H,Cai Y,You Q,Fu H,Shao L H .2022 .Systemic immune-inflammation index is associated with diabetic kidney disease in Type 2 diabetes mellitus patients: Evidence from NHANES 2011-2018. Endocrinol (Lausanne), 13 : 1071465.
[19] Hong SW,Lee J,Cho JH,Kwon H,Park SE,Rhee EJ,Park CY,Oh KW,Park SW,Lee WY .2018 .Pioglitazone attenuates palmitate-induced inflammation and endoplasmic reticulum stress in pancreatic β-cells. endocrinol. Metab (Seoul), 33(1) : 105-113.
[20] Hsu YW,Tsai CF,Chen WK,Huang CF,Yen CC .2011 .A subacute toxicity evaluation of green tea (Camellia sinensis). extract in mice. Food Chem, 49(10) : 2624-2630.
[21] Hu Q,Zuo T,Deng L,Chen S,Yu W,Liu S,Liu J,Wang X,Fan X,Dong Z X .2022 .β-Caryophyllene suppresses ferroptosis induced by cerebral ischemia reperfusion via activation of the NRF2/HO-1 signaling pathway in MCAO/R rats. Phytomedicine, 102 : 154112.
[22] Hu YC,Hao DM,Zhou LX,Zhang Z,Huang N,Hoptroff M,Lu YH M .2014 .- dimethylchalcone protects the impaired insulin secretion induced by glucotoxicity in pancreatic β-cells. J. Agric. Food Chem, 62(7) : 1602-1608.
[23] Mukhtar H Khan N .2018 .Tea polyphenols in promotion of human health. Nutrients, 11(1) : 39.
[24] Khdair SI,Al-Naimat OS,Jarrar W,Al-Qerem W,Khudeir FA .2023 .The influence of TNF-α, IL-6, TGFβ1, IFN-γ, IL-10 polymorphisms on predisposition to diabetes mellitus among Jordanian patients. Endocr Metab Immune Disord Drug Targets, 23(5) : 681-691.
[25] Kornelius E,Li HH,Peng CH,Yang YS,Chen WJ,Chang YZ,Bai YC,Liu S,Huang CN,Lin CL .2019 .Liraglutide protects against glucolipotoxicity-induced RIN-m5F β-cell apoptosis through restoration of PDX1 expression. J. Cell Mol. Med, 23(1) : 619-629.
[26] Lee Y S and Olefsky J. .2021 .Chronic tissue inflammation and metabolic disease. Genes Dev., 35(5- 6) : 307-328.
[27] Lenzen S .2008 .Oxidative stress: the vulnerable beta-cell. Biochem. Soc. Trans., 36 : 343-347.
[28] Li J,Wu N,Chen X,Chen H,Yang X,Liu C .2019 .Curcumin protects islet cells from glucolipotoxicity by inhibiting oxidative stress and NADPH oxidase activity both in vitro and in vivo. , 11(6) : 152-164.
[29] Lin SC,Tsou SH,Kuo CY,Chen WL,Wu KW,Lin CL,Huang CN .2023 .Denosumab attenuates glucolipotoxicity-induced β-Cell dysfunction and apoptosis by attenuating RANK/RANKL Signals. Int. J. Mol. Sci, 24(12) : 10289.
[30] Loboda A,Damulewicz M,Pyza E,Jozkowicz A,Dulak J .2016 .Role of Nrf2/HO-1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism. Cell Mol. Life Sci, 73(17) : 3221-3247.
[31] Lv C,Cheng T,Zhang B,Sun K,Lu K K .2023 .Triptolide protects against podocyte injury in diabetic nephropathy by activating the Nrf2/HO-1 pathway and inhibiting the NLRP3 inflammasome pathway. Ren, 45(1) : 2165103.
[32] Lytrivi M,Castell AL,Poitout V,Cnop M .2020 .Recent insights into mechanisms of β-Cell lipo- and glucolipotoxicity in type 2 diabetes. J. Mol. Biol, 432(5) : 1514-1534.
[33] Ma Q .2013 .Role of nrf2 in oxidative stress and toxicity. Annu. Rev. Pharmacol. Toxicol., 53 : 401-426.
[34] Martínez-Ramírez OC,Salazar-Piña DA,de Lorena RM,Castro-Hernández C,Casas-Ávila L,Portillo-Jacobo JA,Rubio J .2021 .Association of NFκβ, TNFα, IL-6, IL-1β, and LPL polymorphisms with type 2 diabetes mellitus and biochemical parameters in a mexican population. Biochem. Genet, 59(4) : 940-965.
[35] Narasimhan A,Flores RR,Robbins PD,Niedernhofer LJ .2021 .Role of cellular senescence in type II diabetes. , 162(10) : bqab136.
[36] Ndisang JF .2017 .Synergistic interaction between heme oxygenase (HO) and nuclear-factor E2- related factor-2 (Nrf2). against oxidative stress in cardiovascular related diseases. Curr. Pharm. Des., 23(10) : 1465-1470.
[37] Nishimura W,Iwasa H,Tumurkhuu M H .2022 .Role of the transcription factor mafa in the maintenance of pancreatic β-Cells. Int. J. Mol. Sci, 23(9) : 4478.
[38] Novoselova EG,Glushkova OV,Khrenov MO,Lunin SM,Novoselova TV,Parfenuyk SB .2021 .Role of innate immunity and oxidative stress in the development of type 1 diabetes mellitus. peroxiredoxin 6 as a new anti-diabetic agent. Biochem, 86(12) : 1579-1589.
[39] Poitout V,Amyot J,Semache M,Zarrouki B,Hagman D,Fontés G .1801 .Glucolipotoxicity of the pancreatic beta cell. Biochim. Biophys. Acta., : 289-298.
[40] Rayego-Mateos S,Rodrigues-Diez RR,FernandezFernandez B,Mora-Fernández C,Marchant V,DonateCorrea J,Navarro-González JF,Ortiz A,Ruiz-Ortega M .2023 .Targeting inflammation to treat diabetic kidney disease: the road to 2030. , 103(2) : 282-296.
[41] Thorens B .2015 .GLUT2, glucose sensing and glucose homeostasis. , 58(2) : 221-232.
[42] Ti Y,Zhang Y,Ban Y,Wang X,Hou Y,Song Z Y .2022 .Polysaccharide from hemerocallis citrina Borani by subcritical water with different temperatures and investigation of its physicochemical properties and antioxidant activity. Front. Nutr, 9 : 982695.
[43] Tinajero MG,Malik VS .2021 .An update on the epidemiology of type 2 diabetes: A global perspective. endocrinol. Metab Clin. North Am., 50(3) : 337-355.
[44] Wan F,Ma F,Wu J,Qiao X,Chen M,Li W .2022 .Effect of lycium barbarum polysaccharide on decreasing serum amyloid A3 expression through inhibiting NF-κB activation in a mouse model of diabetic nephropathy. (Amst), 7847135 : .
[45] Wan W,Qin Q,Xie L,Zhang H,Wu F,Stevens R C and Liu Y F .2023 .GLP-1R signaling and functional molecules in incretin therapy. Molecules, 28(2) : 751.
[46] Wang Y,Gao L,Chen J,Li Q,Huo L,Wang Y,Wang H,Du J. .2021 .Pharmacological modulation of Nrf2/HO1 signaling pathway as a therapeutic target of parkinson's disease. Front. Pharmacol., 12 : 757161.
[47] Wang Y,Li Y,Liu Y,Chen X,Wei X .2015 .Extraction, characterization and antioxidant activities of Seenriched tea polysaccharides. Int. J. Biol. Macromol, 77 : 76-84.
[48] Wu S,Zhu J,Wu G,Hu Z,Ying P,Bao Z,Ding Z,Tan X .2022 .6-Gingerol alleviates ferroptosis and inflammation of diabetic cardiomyopathy via the Nrf2/HO-1 pathway. Oxid. Med, : 3027514.
[49] Xia ZH,Jiang X,Li K,Li LX,Chen WB,Wang YX,Liu YQ .2020 .Curcumin inhibits alloxan-induced pancreatic islet cell damage via antioxidation and antiapoptosis. J. Biochem. Mol. Toxicol, 34(7) : e22499.
[50] Xiang G,Sun H,Chen Y,Guo H,Liu Y,Li Y,Lu C,Wang X. .2023 .Antioxidant and hypoglycemic activity of tea polysaccharides with different degrees of fermentation. Int. J. Biol. Macromol, 228 : 224-233.
[51] Yan Q,Zhang H,Ma Y,Sun L,Chen Z,Zhang Y,Guo W Y .2025 .AQP1 mediates pancreatic β cell senescence induced by metabolic stress through modulating intracellular H2O2 level. Free Radic. Biol. Med, 226 : 171-184.
[52] Yan Y,Ren Y,Li X,Zhang X,Guo H,Han Y,Hu J .2018 .A polysaccharide from green tea (Camellia sinensis L.) protects human retinal endothelial cells against hydrogen peroxide-induced oxidative injury and apoptosis. Int. J. Biol. Macromol, 115 : 600-607.
[53] Yang G,Liang X,Hu J,Li C,Hu W,Li K,Chang X,Zhang Y,Zhang X,Shen Y,Meng X Y .2022 .Feeding tea polysaccharides affects lipid metabolism, antioxidant capacity and immunity of common carp (Cyprinus carpio L.). Front. Immunol., 13 : 1074198.
[54] Yao J,Liu H,Ma C,Pu L,Yang W .2022 .A review on the extraction, bioactivity and application of tea polysaccharides. Molecules, 27(15) : 4679.
[55] Yousef AI,Shawki HH,El-Shahawy AA,El-Twab SMA,Abdel-Moneim A,Oishi H A .2021 .Polydatin mitigates pancreatic β-cell damage through its antioxidant activity. Biomed, 133 : 111027.
[56] Zhao T,Li C,Wang S,Song X. .2022 .Green tea (Camellia sinensis): A review of its phytochemistry, pharmacology and toxicology. Molecules, 27(12) : 3909.
[57] Zhou P,Xie M,Nie S,Wang X .2004 .Primary structure and configuration of tea polysaccharide. , 47(5) : 416-424.
[58] Zhou Y,Xiang Y,Liu S,Li C,Dong J,Kong X,Ji X,Cheng X .2024 .RIPK3 signaling and its role in regulated cell death and diseases. Cell Death Discovery, 10(1) : 200.
Citations
Citations are not available for this document.
0

Citations

0

Downloads

5

Views