Abstract:
Sclerotium rolfsii is a soil born pathogen. It is very destructive pathogen that affects a number of crops, vegetables and fruits. The most serious impact of the fungus is that it produces sclerotia that can remain in soil for many years and can affect a number of crops in the following seasons. Control of such destructive soil born pathogen is difficult. Different disease management techniques can be used to control collar rot disease; however, fungicides are the most frequently employed. Chemical fungicides, cause environmental damage, health risks, pest resurgence, development of pathogen resistance, extinction of non-target species, and degradation of natural habitats. Present study designed to manage the white mycelia disease known as collar rot by using methanolic seed extract of Zanthoxylum armatum. The goal of the present study was to determine the antifungal properties of seeds of Zanthoxylum armatum and to check the effectiveness of this metanolic seed extract against Sclerotium rolfsii. Malt extract broth was made with various concentrations of methanolic seed extract and DMSO including (50 mg/ml, 25, 12.5, 6.25, 3.125 and 1.562). All concentrations of DMSO and malt extract broth significantly decrease the fungal fresh biomass over control. Gas chromatography-mass spectrometry (GC-MS) analysis of methanol seed extract of Zanthoxlum armatum determines the possible antifungal constituents. Methanol fraction of seeds contained 15 compounds. These compounds belong to ester, alkene, alcohol, carboxylic acid and arene groups. Among the isolated compounds n-hexadecanoic acid, Oleic acid, and hexadecanoic acid methyl ester have antifungal activity. N-hexadecanoic acid was found in the highest percentage (44.00%) followed by oleic acid (17.84%) and hexadecanoic acid, methyl ester (5.88%). Literature survey showed that some of the identified compounds possess antifungal activities against various fungal species and could be responsible for antifungal activity
Page(s):
140-140
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
Antifungal
,
seed extract
,
Zanthoxylum armatum
,
GCMS analysis