Abstract:
Isolation and identification of possible probiotics was carried out from DZO (Bos grunniens × Bos primigenius) milk. DZO is hybrid between domestic cow and yak. There were 14 pure colonies isolates which were subjected for further investigation. Isolation, phenotypic identification, Biochemical microscopic and molecular test were performed to identify the isolates. All 14 isolates were catalase and oxidase negative. Out of 14, 4 of them were cocci and other 10 were gram positive bacilli. 3 isolates were chosen for some specific test including bile tolerance test, acid tolerance test, hemolysis, antibiotic susceptibility and antimicrobial activity against different pathogenic bacteria, 3 isolates were found and describe for the evaluation of their probiotic characteristics. These 3 isolates were shown to be tolerate bile salt an acidic pH. The isolates were amplified using 16s ribosomal RNA by utilizing specific primer for Lactic acid bacteria i.e., primer (27F and 1492R). An automated DNA sequencer were used to obtain PCR product. To find the similar sequence with other genes in the Gene-Bank database (NCBI) and BLAST search to find closely related sequence. MEGA 11.0 was used to construct phylogenetic tree of an identified strain. All the isolates were related to lactic acid bacteria and had 96 to 99% homology for the 16s ribosomal RNA. Based on observed data, it was determined that all 3 isolates were fulfill the requirement for probiotic ability. Specific gene sequence was used to identify the strains, two of strains were Latilactobacillus sakei and one was Lactococcus lactis were shown as a new probiotic in this research.
Page(s):
302-302
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
lactic acid bacteria
,
probiotics
,
DZO
,
16s ribosomal RNA
,
Latilactobacillus sakei