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The combined use of five bacteria including bacillus amyloliquefaciens Y1 as biofertilizer in compost improved low bush blueberry growth, rhizosphere bacteria and enzyme at various pH.
Author(s):
1. Qaiser Jamal: Department of Microbiology, Kohat University of Science and Technology,Kohat,Pakistan
2. Yong Seong Lee: Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University,Gwangju 61186,South Korea
3. Shahzad Munir: Faculty of Plant Protection, Yunnan Agricultural University,Kunming 650201, Yunnan,China
4. Kil Yong Kim: Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University,Gwangju 61186,South Korea
5. Muhammad Saqib Malik: Department of Microbiology, Kohat University of Science and Technology,Kohat,Pakistan
Abstract:
The present study was performed to investigate blueberry compost material alternative to commercially available one mainly containing peat moss and evaluate its potential in pot experiment using five bacteria. Initially, we divided our made compost (M) into three types based on pH (4.5, 5.5 and 7) using H2SO4. In physiochemical analysis, each type of M was found to have different composition of N P, Ca, Mg, Mn and S and higher than commercial compost. Our results indicate negative effects of acidification on bacterial population, diversity and activities of protease, chitinase, and cellulase in M compost. In blueberry pot experiment, plants were grown in 4.5 M, 5.5 M, 4.5 C and 7 M. Two types of treatments were performed including chemical fertilizer (F) and the other type contained bacterial culture (C) including B. amyloliquefaciens Y1, B. licheniformis MH48, B. pumilus L1, P. ehmensis KWN38 and P. elgii HOA73 grown together in broth containing chitin powder. The use of biofertilizers significantly increased plant biomass compared to all fertilizer treatments and pH 7 M treated with bacterial culture (C) has highest value among all treatments. Inoculation of bacteria not only increased total number and type of bacteria but also improved chitinase and gelatinase in rhizosphere of blueberry plants. These results suggest that our made compost can be used as blueberry compost and inoculation of five bacteria have positive effects on blueberry growth, bacterial population and enzyme activities in compost at various pH.
Page(s): 409-416
Published: Journal: Pakistan Journal of Agricultural Sciences, Volume: 55, Issue: 2, Year: 2018
Keywords:
compost , blueberry , bacteria , biofertilizers , crab shell powder , chitinase
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