An in vitro study on micropropagation of caladium bicolor. | [International Journal of Agriculture and Biology • 2007]

Author(s):

1. Aamir Ali: Seed Centre, University of the Punjab, Quaid-e Azam Campus, Lahore, Pakistan

2. Asifa Munawar: Department of Botany, Lahore College for Women University, Lahore, Pakistan

3. Shagufta Naz: Department of Botany, Lahore College for Women University, Lahore, Pakistan

Abstract:

Rapid propagation of Caladium bicolor was achieved in vitro. Excellent results for shoot induction from apical meristem were obtained when it was cultured on MS medium containing I mg L-1 BAP within 8 days of inoculation. For shoot multiplication maximum number of shoots was obtained when 0.25 mg L-1 NAA was added to shoot induction medium i.e., MS medium supplemented with I mg L-1 BAP + 0.25 mg L-1 NAA. Cent percent rooting was achieved by transferring an individual microshoot to modified MS medium containing combination of 2 mg L-1 IBA + 1 mg L-1 NAA. Plantlets were successfully transferred to greenhouse in sterilized sand and nourished with Hoagland`s solution. After acclimatization these plants were shifted to natural conditions in pots containing growth mixture (sand + clay + peat at 1:1:1 ratio), where 100% survival was observed. We concluded that technique of micropropagation can successfully be used for large scale production of premium gtmlity planting material and almost 2500 plants of Caladium could be generated from single apical meristem in a 12 weeks period.

Page(s): 731-735

DOI: DOI not available

Published: Journal: International Journal of Agriculture and Biology, Volume: 9, Issue: 5, Year: 2007

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