Abstract:
Periploca aphylla Decne. belongs to the family Asclepiadoideae. In folk medicine, the milky juice of P. aphylla is externally applied to swellings and tumors. Brine shrimp lethality assay mostly associate well with antitumor and cytotoxic properties. In this study, the cytotoxic potentials of various fractions of crude methanolic extract of P. aphylla were investigated. The cytotoxic activities of different fractions of P. aphylla were determined by using brine shrimp assay. Results: The n-hexane fraction showed low cytotoxicity (53.33%) at 1000 mg/ml, while 33.33 and 20% was observed at 100 and 10 mg/ml, respectively. The LD 50 value calculated was 736.396 mg/ml for hexane fraction. The chloroform fraction marked good activity at all concentrations (63.33, 56.66 and 33.33 at 1000, 100 and 10 mg/ml, respectively). The LD 50 was 84.91 mg/ml. The ethyl acetate fraction exhibited good brine shrimp lethality at respective test concentrations that was 76.66, 70 and 30% at 1000 mg/ml, 100 mg/ml and 10 mg/ml, respectively. The LD 50 value calculated was 41.599 mg/ml. The butanol fraction of the plant exhibited good cytotoxic activity (83.33, 60 and 30%) at respective test concentrations. The LD 50 value calculated was 51.10 mg/ml. The aqueous fraction showed good cytotoxic activity (90, 70 and 30%) at respective concentrations and the LD 50 was 35.93 mg/ml. Conclusion: Hence, it can be concluded from these results that P. aphylla possess strong cytotoxic potential which justifies its traditional use against tumors
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022