Tracking of the antibiofilm activities of lakum leaf extract (Causonis trifolia Linn.) against Staphylococcus aureus | [Pakistan Journal of Biological Sciences • 2023]

Author(s):

1. Lutfi Chabib: Department of Pharmacy, Islamic University of Indonesia, Sleman Regency, Special Region of Yogyakarta 55584, Indonesia

2. Hasyrul Hamzah: Faculty of Pharmacy, University of Muhammadiyah Kalimantan Timur Samarinda, Kota Samarinda, Kalimantan Timur 75124, Indonesia; Indonesian Biofilm Research Collaboration Center, Yogyakarta 55281, Indonesia

3. Widya Rahmah: Faculty of Pharmacy, University of Muhammadiyah Kalimantan Timur Samarinda, Kota Samarinda, Kalimantan Timur 75124, Indonesia; Indonesian Biofilm Research Collaboration Center, Yogyakarta 55281, Indonesia

4. Suci Fitriani Sammulia: Program Study of Pharmacy, Health Institute of Mitra Bunda, Kota Batam, Kepulauan Riau 29444, Indonesia

5. Endang Setyowati: Faculty of Health Science, Pharmacy Study Program, University Muhammadiyah of Kudus, Kabupaten Kudus, Jawa Tengah 59316, Indonesia

6. A. Nurfitriani: Faculty of Agriculture, University Ichsan Gorontalo , Kota, Gorontalo 96138, Indonesia

Abstract:

Background and Objective: Biofilms as a bacterial defense are relatively more difficult to eradicate with antibiotics, thus pathogenic bacteria in their biofilm form can cause serious problems for human health. Lakum (Causonis trifolia L.) is an herbaceous plant with many biological activities, one of which is an antimicrobial compound containing flavonoids, squalene, nimbidin, saponins, anthocyanins, tannins, myricetin, others. This study aimed to determine the antibiofilm activity of Lakum leaf extract against Staphylococcus aureus bacteria and the active compounds that play a role in inhibiting monomicrobial biofilms. Materials and Methods: This research method was carried out with an in vitro experimental study design using observations of phytochemical screening test results and the effectiveness of Lakum leaf antibiofilm on Staphylococcus aureus through microplate reader readings that measure optical density values. Results: This study showed that Lakum leaves contain alkaloids, flavonoids, phenolics, polyphenols, tannins and saponins. In addition, Lakum leaves gave biofilm inhibitory activity in the middle and maturation phase with the highest concentration in 1% extract of 76.95±0.0007 and 72.85± 0.0003%, respectively. Meanwhile, the lowest concentration was 0.125% extract of 65.65±0.0001% in the middle phase and 59.71±0.0003% in the maturation phase. Conclusion: That Lakum leaves have biofilm inhibitory activity on Staphylococcus aureus with flavonoid compounds, tannins and polyphenols that work as active substances in inhibiting the biofilm formation.

Page(s): 91-100

DOI: 10.3923/pjbs.2023.91.100

Published: Journal: Pakistan Journal of Biological Sciences, Volume: 26, Issue: 2, Year: 2023

Keywords:

antibiofilm , biofilm , Causonis trifolia , Lakum leaf , CAUTI , resistant

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