Abstract:
A precise, simple and rapid high performance liquid chromatographic (HPLC) method for the determination and quantification of Levocetrizine has been developed. The chromatographic system consisted of a shimadzu LC-10ATVP pump, SPD-10AVP UV / Visible detector and autosampler (SIL 20A). Separation was achieved on the µ Bondapak C18 125A0 10µm column at room temperature. The sample was introduced through an injector valve of autosampler with a 10µl sample loop, Buffer: Acetonitril (58 : 42 v/v) was used as mobile phase, with flow rate of 1.2ml / minute. PH was adjusted 2.4 with 1N sulfuric acid. UV detection was performed at 230nm. The obtained result showed a good agreement with declared content. Recovery values of Levocetrizine dihydrochloride in tablet (In Xyazl 05mg tablets) were from 99.88 % to 100.08%. The puroposed method is rapid, accurate and selective; it may be used for the quantitative analysis of Levocetirizine dihydrochloride from raw material, in bulk drugs and other dosage formulation.
Page(s):
32-36
DOI:
DOI not available
Published:
Journal: Karachi University Journal of Science, Volume: 39, Issue: 0, Year: 2011