The Inhibitory effect of the Plant Alkaloid Camptothecin on the Rice Sheath Blight Pathogen Rhizoctonia solani. | [International Journal of Agriculture and Biology • 2017]

Author(s):

1. Ting Ting Dai: Southern China Collaborative Innovation Center of Sustainable Forestry, College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu 210037, China

2. Zhiye Xu: Southern China Collaborative Innovation Center of Sustainable Forestry, College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu 210037, China

3. Xiang Zhou: National Joint Local Engineering Laboratory of Biopesticide High-efficient Preparation, School of Forestry and Biotechnology, Zhejiang A & F University, 311300, Lin-an, China

4. Bin Li: National Joint Local Engineering Laboratory of Biopesticide High-efficient Preparation, School of Forestry and Biotechnology, Zhejiang A & F University, 311300, Lin-an, China: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, China

5. Sheng Feng Mao: National Joint Local Engineering Laboratory of Biopesticide High-efficient Preparation, School of Forestry and Biotechnology, Zhejiang A & F University, 311300, Lin-an, China: Southern China Collaborative Innovation Center of Sustainable Forestry, College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu 210037, China

Abstract:

The rice sheath blight caused by Rhizoctonia solani is one of the most destructive diseases of rice worldwide. Present study presented the inhibitory effect and mechanism of the plant alkaloid camptothecin on R. solani. Results indicated 14.32, 34.88, 59.94, 68.54, 76.38 and 77.72% inhibition of mycelial growth after 48 h of incubation at 6.25, 12.50, 25.00, 50.00, 100 and 200 µg/mL camptothecin. The soluble protein content in mycelia was significantly increased, the N-acetyl glucosamine content and chitinase activity was significantly reduced, but the sugar content was unaffected by camptothecin at 100 µg/mL after 12 h and 24 h treatment. Moreover, the optical microscopic and transmission electron microscopic observation revealed that the fungal mycelia are sparse with a loose structure, mitochondrial nule3mber, and morphological characteristics of camptothecin-treated R. solani. Overall, the antifungal activity of camptothecin against R. solani, suggested that camptothecin has a great potential in the control of rice sheath blight.

Page(s): 558-562

DOI: 10.17957/IJAB/15.0335

Published: Journal: International Journal of Agriculture and Biology, Volume: 19, Issue: 3, Year: 2017

Keywords:

Keywords are not available for this article.

References:

[1] Akhtar , J.,V.K.Jha,A.Kumar and H.C. Lal, 2009.Occurrence of banded leaf and sheath blight of maize in Jharkhand with reference to diversity in Rhizoctonia solani,Asian J. Agric. Sci. 1 32 -35

[2] Bradford , M.M., 1979.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,Anal. Biochem 72 248 -254

[3] Ciampi , M.B.,M.J.N.Costa,M.Zala,B.A.McDonald,P.C.Ceresini, 2008.Genetic structure of populations of Rhizoctonia solani anastomosis group-1 IA from soybean in Brazil, 98 932 -941

[4] Fukuoka , M.,H.Niitani,S.Suzuki,M.Motomiya,K.Hasegawa,Y.Nishiwaki,T.Kuriyama,Y.Ariyoshi,S.Negoro,N.Masuda,S.Nakajima,T.Taguchi, 1992.A phase II study of CPT-11, a new derivative of camptothecin, for previously untreated non-small-cell lung cancer,J. Clin. Oncol 10 16 -20

[5] Hsiang , Y.H.,R.Hertzberg,S.Hecht,L.F.Liu, 1985.Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase,J. Biol. Chem 260 14873 -14878

[6] Lerchen , H.G.,J.Baumgarten, 2001.Design and optimization of 20-Olinked camptothecin glycoconjugates as anticancer agent,J. Med 44 4186 -4195

[7] Li , B.,Y.Shi,C.L.Shan,Q.Zhou,M.Ibrahim,Y.L.Wang,G.X.Wu,H.Y.Li,G.L.Xie,G.C.Sun, 2013.Effect of chitosan solution on the inhibition of Acidovorax citrulli causing bacterial fruit blotch of watermelon,J. Sci. Food 93 1010 -1015

[8] Li , C.J.,C.R.Gong,R.Li,X.W.Lin, 2000.Studies on the metabolism of protein and nitrate nitrogen in tobacco leaf during curing,J. Henan Agric 34 47 -49

[9] Liu , H., W.X.,Tian , B.,Li , G.X.,Wu , M.,Ibrahim , Z.Y.,Tao , Y.L.,Wang , G.L.,Xie , H.Y.,Li and G.C.,Sun, 2012.Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen, Rhizoctonia solani, 34 2291 -2298

[10] Padaria , J.C.,A.Singh, 2009.Molecular characterization of soil bacteria antagonistic to Rhizoctonia solani, sheath blight of rice,J. Environ. Sci. Heal B. 44 397 -402

[11] Pucci , S.,M.J.Zonetti,T.Fisco,C.Polidoro,G.Bocchiufuso,A.Palleschi,G.Novelli,L.G.Spagnoli,P.Mazzarelli, 2016.Carnitine palmitoyl transferase-1A (CPT1A): a new tumor specific target in human breast cancer[J],Oncotarget 7 19982 -19996

[12] Rush , M.C.,F.N.Lee,R.K.,P.S., 1992.Sheath blight,In: Compendium of Rice Diseases 22 -23

[13] Reinke , S.N.,X.Hu,B.D.Sykes,B.D.Lemire, 2010.Caenorhabditis elegans diet significantly affects metabolic profile, mitochondrial DNA levels, lifespan and brood size,Mol. Genet 100 274 -282

[14] Sánchez-Ferrer , A.,J.N.Rodríguez-López,F.García-Cánovas,F.García-Carmona, 1995.Tyrosinase: a comprehensive review of its mechanism,Biochim. Biophys. Acta 1247 1 -11

[15] Sirikantaramas , S.,M.Yamazaki,K.Saito, 2008.Mutations in topoisomerase I as a self-resistance mechanism coevolved with the production of the anticancer alkaloid camptothecin in plants,Proc. Natl. Acad. Sci. USA 105 6782 -6786

[16] Subramaniana , N.,N.Sundaraganesan,S.Sudha,V.Aroulmoji,G.Sockalingame and M. Bergamin, 2011.Experimental and theoretical investigation of the molecular and electronic structure of anticancer drug camptothecin,Spectrochim. Acta A. 78 1058 -1067

[17] Su , H.F.,L.Zhang,Y.L.Cao,Y.B.Li, 2009.Pretreatment of citrus fruit dregs with white-rot fungi for anaerobic digestion and methane production,J. Southwest Univ. 12 71 -76

[18] Thomas , C.J.,N.J.Rahier,S.M.Hecht, 2004.Camptothecin: current perspectives,Bioorg. Med 12 1585 -1604

[19] Vogel , C.,A.Kienitz,R.Muller,H.Bastians, 2005.The mitotic spindle checkpoint is a critical determinant for topoisomerase-based chemotherapy,J. Biol. Chem 280 4025 -4028

[20] Wang , Y.L.,S.Y.Liu,X.Q.Mao,Z.Zhang, 2013.Identification and characterization of rhizosphere fungal strain MF-91 antagonistic to rice blast and sheath blight pathogens,J. Appl. Microbiol. 114 1480 -1490

[21] Xiong , Z.Q.,X.R.Tu,S.J.Wei,H.Lin, 2013.The Mechanism of antifungal action of a new polyene macrolide antibiotic antifungalmycin 702 from streptomyces padanus JAU4234 on the rice sheath blight pathogen Rhizoctonia solani,PLOS One 8 73884 -

[22] Yang , Y.J.,H.Dong,X.Xu, 2013.The status and advance in research of botanical fungicide,J. Hebei Agric. Sci. 12 53 -57

Citations

Downloads

Views