A study on genetic diversity of Cuscuta reflexa roxb. And few members of Convolvulaceae on the basis of RAPD and SDS-PAGE | [Pakistan Journal of Botany • 2021]

Author(s):

1. QUMQUM NOSHAD: Department of Botany Mirpur University of Science and Technology (MUST),Mirpur-10250(AJK),Pakistan

2. MUHAMMAD AJAIB: Department of Botany Mirpur University of Science and Technology (MUST),Mirpur-10250(AJK),Pakistan

3. AYSHA KIRAN: Department of Botany, University of Agriculture Faisalabad, Pakistan

4. MUHAMMAD ISHTIAQ: Department of Botany Mirpur University of Science and Technology (MUST),Mirpur-10250(AJK), Pakistan

5. TANVEER BASHIR: Department of Botany Mirpur University of Science and Technology (MUST), Mirpur-10250(AJK), Pakistan

6. MUHAMMAD FAHEEM SIDDIQUI: Department of Botany, University of Karachi,Karachi 75270,Pakistan

Abstract:

In the research, genetic diversitry of Cuscuta refelexa Roxb.and some slected taxa of Convolovulaceae was conducted using DNA markers and SDS-PAGE technique. Four primers were used for the amplification of genomic DNA of twelve samples of 11 species belonging to Convolvulaceae and one species of Cucutaceae. RAPD-PCR was performed by the use of four primers and in total 40 bands were produced and among these 24 bands showed polymorphism. On the basis of pair wise comparison between species, the highest number of polymorphic bands were obtained by primer 4, band size generated by these primers ranged from 200-500bp. Maximum similarity was observed between Cuscuta reflexa Roxb. and Ipomoea pes-tigridis L. with 100 similarity index at distance level of 1.375. Same plant samples were also evaluated for their total protein variability. Protein was extracted and loaded on polyacrylamide gel for electrophoresis. Observed proteins bands were ranged in size from 20 to 200 kDa. C. reflexa has only 20 and 40 kDa protein that is found in every studied member of Convolvulaceae. Maximum similarity was observed between C. reflexa and Ipomoea pes-tigridis with 100% at distance level of 0.355. A significant result of this study was the identification of close relationship among C. reflexa Roxb. and Ipomoea pes-tigridis L. confirmed by both SDS and RAPD analysis.

Page(s): 959-965

DOI: 10.30848/PJB2021-3(13)

Published: Journal: Pakistan Journal of Botany, Volume: 53, Issue: 3, Year: 2021

Keywords:

RAPD , SDSOAGE , Cuscutaceae , Convolvulaceae , Cuscuta reflexa Roxb , Ipomoea species

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