Author(s):
1. Muhammad Usman Aslam:
Institute of Botany, University of the Punjab Lahore, Pakistan
2. Zahoor Ahmad Sajid:
Institute of Botany, University of the Punjab Lahore, Pakistan
Abstract:
This study was conducted to investigate the regeneration potential of Crocus sativus (Saffron) of the family Iridaceae which is an economically important plant being used in the food, perfumery, medicine, and cosmetics industry. Segments of leaves and corms of Saffron were cultured as explants on MS media fortified with different concentrations of auxins and cytokinins. Maximum callus induction was observed when these explants were cultured on MS medium supplemented with 13.56 µM 2,4-D. The callus obtained from leaves was friable in texture with no potential for regeneration which becomes necrotic after 28 days. Whereas the callus obtained from corms was compact and dark brown in color which had the capability of regeneration. The results of this investigation revealed that the response of 2,4-D was efficient when used in high concentration and favour the callus formation. Shoot regeneration was significantly influenced by the type of PGRs used in the medium and MS medium supplemented with 26.85 µM NAA + 22.7 µM TDZ exhibited the highest (100%) shoot regeneration with three leaves. MS medium containing 2.26 µM 2,4-D + 4.44 µM BAP + 5.70 µM IAA was found to be most suitable for shoot proliferation and elongation.
Page(s):
32-32
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
Regeneration
,
Callus
,
Crocus sativus
References:
References are not available for this document.
Citations
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