Abstract:
A new simple ultrafiltration technique which requires only simple apparatus has been described and the procedure outlined for ultrafiltration of blood serum. The technique is unique as unlike all other methods described in the literature it requires only small amounts of serum or plasma to obtain enough ultrafiltrate for further analysis. The importance of the method of sample collection, duration of ultrafiltration and pH was investigated. The most important advantage of this technique is the effective control of pH during the ultrafiltration process and this is attained by maintaining a constant atmosphere of 5% carbon dioxide over the specimen which prevents any major change in the pH of the specimen, since ultrafiltration results are considerably effected by large changes in pH. This eliminated any need for collecting blood under oil etc. to prevent changes in pH. The duration of ultrafiltration had no effect on the partition of serum calcium. For twenty healthy human subjects, the range for ultrafiterable calcium was found to be 55-66% of the total serum calcium.
Page(s):
29-36
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Biochemistry, Volume: 11, Issue: 1--2, Year: 1978