Abstract:
Aerial parts of plants termed as phyllosphere are normally colonized by a variety of bacteria, yeasts, and fungi. The aerial inhabitants are called epiphytes. Bacterial colonization as biofilms is well documented and has been reported to cause many economic and health problems. Hence this study was designed to isolate microbes from phyllosphere of Olea europaea (olive plant species) and assess the biofilm forming ability. Four strains L1, L2, L3 and L4 were isolated from phyllosphere of O. europaea and characterized morphologically, biochemically and physiologically. 50% strains were gram positive while 50% was observed to be gram negative. All strains were catalase positive with no H2S production. All strains showed best growth at 37°C temperature and pH 7. From above characterization, strains were found to belong to genera Erwinia spp., Pseudomonas spp., Micrococcus spp. and Bacillus spp. Biofilm formation determined by two methods i.e., Congo red and cover slip proved Bacillus spp. as the only biofilm former Congo red method. Afterwards mutations were induced at different time intervals i.e., 3, 4, and 15 minutes. There was an incredible difference in biofilm formation between wild type and mutant strains. Highly significant biofilm formation (p>0.001) was observed in all mutated strains after 15 minutes of UV exposure. Hence our study discussed the significant decrease in biofilm formation of various pathogenic strains of O. europaea after UV exposure for 15 minutes which could be applied to overcome many plant diseases in future.
Page(s):
151-151
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023