Abstract:
An increase in thrombosis, a fatal side effect of medical conditions that causes myocardial infarction and some other significant cardiovascular disorders, which is frequently caused by the fibrin clots accumulation in the blood vessels. Production of fibrinolytic enzyme was carried out in this research work. Indigenous compost samples were collected. After processing, fungal samples were inoculated on PDA (Peptone Dextrose-Agar media) plates and Fibrin salt basal media was used for isolation of Oidiodendron flavum and bacterial samples were inoculated on Nutrient Agar plates and Fermentation media was used for propagation of Bacillus subtilis. Wheat bran and Rape seed cake were used as substrates for fungal and bacterial strains. The fungal strain (BBT-OF1) and bacterial strain (BBT-BS1) were used for the production, isolation, propagation, purification and optimization of fibrinolytic enzyme. The enzyme produced by the fungal strain resulted in 305 U/ml with 87.14 U/mg specific activity, 3.9 mg/ml protein, 4.4-fold purification and 67% recovery. On the other hand, the enzyme produced by bacterial strain resulted in 310 U/ml with 63.26 U/mg specific activity, 4.9 mg/ml protein, 4.81-fold purification and 82.22% recovery. Fibrinolytic enzyme produced from the isolated strains exhibited its activity within the range of pH (7.0-8.0). It showed its best performance at pH 8.0 for Oidiodendron flavum and at pH 7.0 for Bacillus subtilis. Thermal stability between 30-50? was shown by the produced fibrinolytic enzyme. In current research, enzyme production from Oidiodendron flavum increased up to highest level at 45°C and from Bacillus subtilis increased up to highest level at 37?. The result was revealed that SDS-PAGE showed molecular weight of 33kDa for Oidiodendron flavum and 30kDa for Bacillus subtilis.For this purpose, growth of Oidiodendron flavum and Bacillus subtilis was optimized at various parameters (pH, temperature, substrate concentration and fermentation period). By using these optimized conditions maximum growth of Oidiodendron flavum and Bacillus subtilis would be obtained and good quality fibrinolytic enzyme with cheap cost can be produced at industrial level.
Page(s):
92-92
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Bacillus subtilis
,
Oidiodendron flavum
,
Fibrinolytic Enzyme
,
Thrombolytic Agents