Abstract:
Lipoprotein lipase (LPL) plays a crucial role in the metabolism and transport of lipids. The polymorphism of the LPL gene exon 8 and associations with duck meat quality traits were investigated using PCR-SSCP analysis and DNA sequencing. A novel silent mutation 1251 T>C was first identified located in exon 8 of the LPL gene. Two alleles (T and C) and three genotypes designed as TT, TC and CC were detected in the PCR products. The T allele and TT genotype were dominant in the five breeds. The 1251 T>C locus belonged to moderately informative polymorphism except for the Yinjiang ducks. The genotype distribution of the Xingyi, Jinding and Cherry Valley populations deviated from Hardy–Weinberg equilibrium but not the Sansui and Yinjiang populations. The 1251 T>C locus was significantly associated with meat quality traits except for pH, water holding capacity, intramuscular fat, C14:1, and C15:0. Significant additive effects were detected for C12:0, C14:0, C16:0, C16:1, C17:0, C18:2, C20:1, C20:3, PUFA and EFA, significant dominance effects for shear value, C14:0, C17:0, C18:0, C18:1, C18:3, C20:0, C20:1, C20:2, C20:4 and UFA. The results suggested that the 1251C>G polymorphism might be useful genetic markers in duck breeding to improve the content of beneficial fatty acids.
Page(s):
1459-1464
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Zoology, Volume: 48, Issue: 5, Year: 2016