The Induction of Polyploid Hairy Roots in Petunia hybrida Using Root Transformation of Agrobacterium rhizogenes K599 and Colchicine | [International Journal of Agriculture and Biology • 2020]

Author(s):

1. Taihe Xiang: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China; Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou, China

2. Zhengxian Xu: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China

3. Xinyi Zhu: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China

4. Youhe Lin: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China

5. Jiangshan Li: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China

6. Fazong Long: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China

Abstract:

In general, polyploid plant tends to have larger size, increased amounts of secondary metabolites, and stronger resistance to stress. On the other hand, hairy root has the advantages of rapid propagation and convenience to genetic manipulation. Thus, considering these two beneficial features, we used Agribacterium rhizogenes K599 to infect the leaves of petunia tissue culture seedlings to induce hairy root, and the hairy roots were subsequently treated with 0.05-0.20% colchicine for 12-36 h. Among them, 0.1% colchicine for 24 h treatment resulted in 55.0% survival rate and 26.70% tetraploid induction rate, which led to chromosome doubling events while maintaining a high survival rate. Microscopic observation of root tip cells confirmed that the polyploid hairy roots were mostly tetraploid 4n = 28. This study provides a theoretical basis for obtaining polyploid plants using root transformation and colchicine.

Page(s): 651-654

DOI: 10.17957/IJAB/15.1482

Published: Journal: International Journal of Agriculture and Biology, Volume: 24, Issue: 4, Year: 2020

Keywords:

hairy root , Root transformation , chromosome doubling

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