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Alkaline protease production from industrial waste by bacillus subtilis ml-4
Author(s):
1. Muhammad Gul-Sher: Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
2. Muhammad Nadeem: Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
3. Quratulain Syed: Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
4. Muhammad Irfan: Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
5. Shahjahan Baig: Food and Biotechnology Research Centre, PCSIR Laboratories Complex, Lahore - 54600, Pakistan
Abstract:
The influence of various culture conditions on protease production by Bacillus subtilis ML-4 was studied in the presence of growth medium containing poultry feed waste (5%), K2HPO4 (0.3%), CaCl2 (0.03%) and MgSO4 (0.015%). Maximum protease production (264.25 ± 1.86 U/ml) was observed at initial pH 9 with 3% (v/v) of inoculum size after 48 h of incubation at 37 °C. The alkaline protease was stable over a broad range of temperature (30 to 60 °C) and pH (8 to 11). However, maximum activity (155.45 U/ml) was observed at temperature 50 °C and pH 10.
Page(s): 146-150
DOI: DOI not available
Published: Journal: Pakistan Journal of Scientific and Industrial Research, Volume: 53, Issue: 3, Year: 2010
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