Use of intergeneric cross for production of doubled haploid wheat (Triticum aestivum L.). | [Science, Technology and Development • 2012]

Author(s):

1. M. Ahsan Khan: Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

2. Shadab Shaukat: Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

3. Javed Ahmad: Wheat Research Institute, Ayub Agriculture Research Institute, Faisalabad, Pakistan

4. M. Kashif: Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

5. Abdus Salam Khan: Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan

6. M. Zafar Iqbal: Agricultural Biotechnology Research Institute, Ayub Agriculture Research Institute, Faisalabad, Pakistan

Abstract:

The main purpose of conventional breeding or hybridisation is to bring about homozygosity, for which 6 to 7 years may be required. Wheat and maize crosses have proved to be more efficient in DH lines production than anther culture methods, because of its lower genetic specificity. Doubled haploid technique facilitates the development of homozygous plants within one generation. The system is developed through haploid production, followed by chromosome doubling, to produce homozygous plants in a single generation. For doubled haploid production method wheat and maize crossing system is better than anther culture and ovule culture because maize pollens are highly responsive and produce stable progeny population. Wheat is being used as female parent and maize as a male parent for the production of doubled haploid. Moreover, Silver Nitrate (AgNO3) in tiller culture media can improve the frequency of haploid embryo production in this crossing system. Our result showed that DH production through wheat and maize crossing system was proved to be time saving (2 years) as compared to other conventional breeding methods (6 years).

Page(s): 295-300

DOI: DOI not available

Published: Journal: Science, Technology and Development, Volume: 31, Issue: 4, Year: 2012

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