Abstract:
The chili pepper is an important crop that is grown on a large scale all over the world and has a considerable impact on the global economy. The crop of chili is susceptible to a wide variety of infections, including viruses, which may result in significant losses in yield. So far 65 viruses, including begomoviruses, have infected chili plants worldwide, causing chili leaf curl virus disease. Begomoviruses are the circular DNA plant viruses. The current study was aimed to identify the infectious agent responsible for chili leaf curl disease complex. In order to identify the viruses that infect chili plants, chili fields were surveyed and infected samples were collected. DNA extraction was done through CTAB method and amplified using PCR to identify the causative agents infecting chilies. To assess chili-infecting begomovirus diversity in Sindh and Punjab Provinces, PCR and rolling circle amplification (RCA) were utilized to clone and sequence full-length viruses and satellites from chili plants. Five different begomoviruses were found through sequence analysis to be infecting chilies in Pakistan: Chili Leaf Curl Virus (ChiLCV), Pepper Leaf Curl Lahore Virus (PepLCLV), Tomato Leaf Curl New Delhi Virus (ToLCNDV), Pepper Leaf Curl Bangladesh Virus (PepLCBDV), and Tomato Leaf Curl Palampur Virus (ToLCPaV). Though 250 begomoviruses are infecting the chili crop globally (14 species of begomoviruses). The nucleotide variations at the species level were revealed by DNA alignment for the sequenced molecules and their closely related molecules in GenBank. Additionally, these begomoviruses that infect chili are associated to a variety of different satellites. These satellites contained the alphasatellite (Cotton Leaf Curl Multan alphasatellite) and the beta satellite(Chili Leaf Curl Betasatellite). Analysis of recombination showed that begomoviruses that infect chilli follow a certain pattern of recombination. Recombination was found to happen most often between ChiLCV, PepLCBDV, and PepLCLV. Based on the infectivity tests, it seems that among the viruses from the Old World, DNA-A alone is enough to cause leaf curl disease in tobacco plants. Current research explores the expansion of begomoviruses that infect chilies and the prevalence of chili leaf curl disease in Pakistan.
Page(s):
172-172
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023