β casein Polymorphism in Indigenous and Exotic Cattle Breeds of Pakistan | [Pakistan Journal of Zoology • 2022]

Author(s):

1. Shakila Mumtaz: Department of Livestock Production, University of Veterinary and Animal Sciences,Ravi Campus Pattoki,Pakistan

2. Khalid Javed: Department of Livestock Production, University of Veterinary and Animal Sciences,Ravi Campus Pattoki,Pakistan

3. Muhammad Dawood: Department of Livestock Production, University of Veterinary and Animal Sciences,Ravi Campus Pattoki,Pakistan

4. Muhammad Imran: Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan

5. Asad Ali: Department of Livestock Production, University of Veterinary and Animal Sciences,Ravi Campus Pattoki,Pakistan

6. Nazia Ramzan: Department of Livestock Production, University of Veterinary and Animal Sciences,Ravi Campus Pattoki,Pakistan

Abstract:

Milk composition depends largely on the breed’s genetics and ration fed. Different proteins can be found in milk. Beta-caseins are thought to be more important because some serious health-related issues in humans have been reported with the consumption of A1 milk (mutated casein variant). This study was planned to investigate the polymorphism in the beta-casein gene (CSN2) in Sahiwal (40), American Holstein Friesian (40) and the crossbred (Sahiwal × HF) (50). PCR-RFLP and conformational sequencing were performed to investigate the beta-casein polymorphism. Results of the present study showed that there was not any mutated genotype (A1A1) available in all of the three breeds. All three breeds possess dominant genotype A2A2 with genotypic frequency (0.925, 0.4, 0.64) respectively and we have also found some heterozygous genotypes A1A2 in all breeds with genotypic frequency (0.075, 0.6, 0.36) respectively. Findings of the present study revealed that A2 (the dominant allele) is present with a pretty much higher frequency (0.9625, 0.7, 0.82) in all the three studied breeds and the mutated allele A1 is present with very low allelic frequency (0.037, 0.3, 0.18).

Page(s): 1451-1454

DOI: 10.17582/journal.pjz/20190307030303

Published: Journal: Pakistan Journal of Zoology, Volume: 54, Issue: 3, Year: 2022

Keywords:

PCRRFLP , DNA sequencing , Beta casein , Genetic variations , A1 and A2 Genotype

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