Effect of Freeze-dried Amnion with Human Amniotic Stem Cells Seeding on the Expression of FGF-2 andthe Number of Fibroblasts in Vesicovaginal Fistula: An Animal Study | [International Journal of Veterinary Science • 2023]

Author(s):

1. Wijayanti: Department of Obstetrics and Gynecology, Faculty of Medicine,Universitas Airlangga, Surabaya,Indonesia

2. Eighty Mardiyan Kurniawati: Department of Obstetrics and Gynecology, Faculty of Medicine,Universitas Airlangga, Surabaya,Indonesia

3. Widjiati: Department of Veterinary Anatomy, Faculty of Veterinary Medicine,Universitas Airlangga, Surabaya,Indonesia

4. Budi Iman Santoso: Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia - Dr. Cipto Mangunkusumo Hospital,Jakarta,Indonesia

Abstract:

Surgery is the standard therapy for vesicovaginal fistula. The surgical success rate in these cases ranges from 70100%, and the risk of recurrence is high. The human amniotic membrane is especially being developed for tissue engineering in urogynecology. The study aimed to determine the effect of freeze-dried amnion with human amniotic stem cells seeding on the expression of FGF-2 and the number of fibroblasts in vesicovaginal fistula in New Zealand rabbits. We conducted experimental research with a post-test-only control group design. The female rabbits were divided into three groups, each consisting of 12 rabbits. The specimens taken were subjected to immunohistochemical staining to determine the expression of FGF-2 and hematoxylin-eosin staining to determine the number of fibroblast cells. We analyzed data statistically. The expression of FGF-2 in primary suturing and freeze-dried amnion with human amniotic stem cell seeding was higher than in the other groups (P<0.05). We found the highest fibroblast cells in the primary suturing and freeze-dried amnion with human amniotic stem cell seeding (P<0.05). The use of freezedried amnion with the seeding of human amniotic stem cells affects postoperative wound healing seen from the expression of FGF-2 and the number of fibroblasts. This study can be used as a recommendation for clinical use but needs to be clarified by conducting human studies.

Page(s): 375-381

DOI: 10.47278/journal.ijvs/2022.205

Published: Journal: International Journal of Veterinary Science, Volume: 12, Issue: 3, Year: 2023

Keywords:

Vesicovaginal fistula , Rabbit , fibroblast , Amnion , FGF2

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