Active compounds from curcuma longa and comparison of their effectively induced apoptosis in MCF-7 Cell | [Pakistan Journal of Biological Sciences • 2021]

Author(s):

1. Ronny Lesmana: Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia;Biological Activity Division, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia

2. Susianti: Biological Activity Division, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia Departement of Chemistry; Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia

3. Todia Pediatama,: Biological Activity Division, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia

4. Nova Sylviana: Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia ; Biological Activity Division, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia

5. Yuni Susanti Pratiwi,: Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; Biological Activity Division, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia

6. Hanna Goenawan: Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia;Biological Activity Division, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia

7. Unang Supratman: Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia

Abstract:

Background and Objective: The natural bioactive compounds of Curcuma longa, known as curcuminoids, has been shown to exerts anticancer effects to diverse cancer cell line in vitro, including breast cancer cell line. These curcuminoids consist of curcumin (Cur), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC). Furthermore, there has never been a study to compare the extent of antiproliferative and apoptotic modulation potential between Cur, DMC and BDMC in the breast cancer cell, until now. In the present study, we explore the efficacy among Cur, DMC and BDMC to alters MCF-7 cell viability, which might lead to apoptotic modulation. Materials and Methods: This kind of study was performed in vitro whereby the cells were maintained in an appropriate medium and the anticancer effect of curcuminoids (Cur, DMC and BDMC) was measured by using resazurin-based PrestoBlue cell viability assay. Later, MCF-7 breast cancer cells were cultured in 12 wells plate added with different concentrations of Cur, DMC and BDMC for western blotting analysis. Statistical analysis was performed with GraphPad 8, One-way ANOVA and Student’s t-test. Results: The result showed that Cur, DMC and BDMC inhibiting the proliferation of MCF-7 cells. In the concentration dose of 31.25 µg mLG1, the cell viability in cells treated with Cur is 27%, DMC is 31.5% and BDMC is 46%. The IC50 dose of Cur, DMC and BDMC were 25.63, 29.94 and 36.91 µg mLG1. Conclusion: Cur is more effective in inhibiting proliferation and apoptotic modulation in MCF-7 cells compare to DMC and BDMC. It represents the potential of Cur, DMC and BDMC as adjunctive therapy in treating breast cancer.

Page(s): 35-41

DOI: 10.3923/pjbs.2021.35.41

Published: Journal: Pakistan Journal of Biological Sciences, Volume: 24, Issue: 1, Year: 2021

Keywords:

Breast cancer , Antiproliferative activity , Curcumin , demethoxycurcumin , MCF7 Cell , Bisdemethoxycurcumin

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