CRISPR/Cas9-mediated Genome Editing in Potatoes | [International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts) • 2022]

Author(s):

1. Aneela Yasmeen: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

2. Abdul Qayyum Rao: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

3. Saira Azam: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

4. Ayesha Latif: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

5. Naila Shahid: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

6. Allah Bakhsh: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

7. Sana Shakoor: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

8. Sara Ajmal: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

9. Sahar Sadaqat: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

10. Momna Muhammad: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore,

11. Muhammad Awais: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

12. Abdul Raouf: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

13. Mehwish Imtiaz: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

14. Tayyab Husnain: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

15. Kausar Malik: Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan

Abstract:

CRISPR-Cas9 is a state of art technology that has enabled researchers to edit genomes. The present study was conducted to investigate the efficiency of genome editing in locally grown potato variety (AGB purple) in comparison to a diploid variety (M6). The stem internodes of 5-6 weeks old potato plants of both varieties were infected with Agrobacterium strain LBA4400 harboring CRISPR-Cas9 vectors expressing a particular guide RNA against vacuolar invertase; and Cas9 endonuclease. Both potato varieties showed encouraging regenerative response on selection media. The overall transformation efficiency of potatoes was found to be 11.7% whereas the indel% was found to be 6 for diploid and 5 for tetraploid variety via Sangers’ sequencing data. The regenerated plantlets were shifted to culture tubes and then to pots after development of proper roots and shoots. The molecular analysis confirmed the integration of cassette and expression of Cas9 in primary transformants. Realtime and Sanger’s Sequencing results concluded that the CRISPR/cas9 can be efficient tool for editing of plants that can be further used in an efficient potato breeding program.

Page(s): 182-182

DOI: DOI not available

Published: Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022

Keywords:

genome editing , CRISPRCas9

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