The investigation of plasma glucose-6-phosphate dehydrogenase, 6-phoshogluconate dehydrogenase, glutathione reductase in premenauposal patients with iron deficiency anemia. | [Pakistan Journal of Medical Sciences • 2014]

Author(s):

1. Fatih Ozcicek: Department of Internal Medicine, Erzincan University, Medicine Faculty, Erzincan, 24100, Turkey

2. Mehmet Aktas: Biochemistry Department, Erzincan University, Medicine Faculty, Erzincan, 24100, Turkey

3. Kultigin Türkmen: Department of Internal Medicine and Nephrology, Erzincan University, Medicine Faculty, Erzincan, Turkey

4. T Abdulkadir Coban: Biochemistry Department, Erzincan University, Medicine Faculty, Erzincan, 24100, Turkey

5. Murat Cankaya: Science and Art Faculty, Biology Department, Erzincan University, Erzincan, Turkey

Abstract:

Background and Objective: Iron is an essential element that is necessary for all cells in the body. Iron deficiency anemia (IDA) is one of the most common nutritional disorders in both developed and developing countries. The glutathione pathway is paramount to antioxidant defense and glucose-6-phosphate dehydrogenase (G6PD)-deficient cells do not cope well with oxidative damage. The goal of this study was to check the activities of G6PD, 6-phosphogluconate dehydrogenase, glutathione reductase in patients with IDA. Methods: We analyzed the plasma samples of 102 premenopausal women with IDA and 88 healthy control subjects. Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activity as compared to the reduction of NADP +, glutathione reductase activity was performed based on the oxidation of NADPH. 2 ml of plasma were used in all analyzes. SPSS program was used for all of the statistical analysis. Result: Diagnosis of iron deficiency in patients belonging to the analysis of blood were ferritin 3.60 ± 2.7 ng / mL, hemoglobin 9.4 ± 1.5 mg / dl and hematocrit 30.7 ± 4.1% ratio; in healthy subjects ferritin 53.5 ± 41.7 ng/ml, hemoglobin level 13.9 ± 1.3 mg / dl and hematocrit ratio 42 ± 3.53%. When compared to healthy subjects the glutathione reductase level (P Conclusion: It was shown that Glucose-6-Phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase have no effect on iron-deficiency anemia in patients. The plasma GR levels of premenopausal women with IDA were found to be higher compared to healthy subjects, which could be secondary to erythrocyte protection against oxidative stress being commonly seen in IDA.

Page(s): 809-813

DOI: DOI not available

Published: Journal: Pakistan Journal of Medical Sciences, Volume: 30, Issue: 4, Year: 2014

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