Abstract:
Antibiotics resistance is rising day by day. Microorganisms increased their resistance to antibiotics present in market which is a main health problem because antibiotics losing their effectiveness at an alarming rate. Treatment of infections caused by resistant microorganisms is quickly becoming one of world's biggest health-care concerns. Nature is the major medicinal source for treatment of broad range of diseases. With the beginning of 19th century, new approaches for discoveries of bioactive compounds started and these bioactive compounds are known as natural products. Microalgae present in nature are reservoir of natural source with huge importance which are not fully discovered until now, that makes way towards the discovery of natural products that have pharmaceutical importance. The aim is to discover bioactive metabolites and assessment of antimicrobial ability of bioactive metabolites from green microalgae Closteriopsis acicularis as well as investigation of biological activities like antioxidant, total phenolic content, and total flavonoid content. In this present study the test organisms' indigenous green microalgae were collected dried and grinded. The dried and grinded samples were treated with different solvents. The antimicrobial activity of crude extract of different solvents were tested against 6 ATCC pathogenic strains of bacteria Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, E. coli, Salmonella enterica and Pseudomonas aeruginosa, and 2 fungal strains Candida albicans and Aspergillus niger by agar well diffusion method. Ethanolic extract shows better results at a concentration of 1mg/ml, then through column chromatography purification of ethanolic extract was done and fractions were tested for antimicrobial activities. The Characterization for validation of pure compound was done by TLC, FTIR, GC MS and NMR. The compounds which were confirmed from this analysis were ester compounds which were responsible for antimicrobial and antioxidant activity.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
metabolites
,
antioxidant
,
antimicrobial
,
Microalgae